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首页> 外文期刊>Applied and Environmental Microbiology >Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate
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Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate

机译：伪装从假单胞菌分离铬酸盐，该假单胞菌被铬酸砷化铜污染的部位

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摘要

A pseudomonad (CRB5) isolated from a decommissioned wood preservation site reduced toxic chromate [Cr(VI)] to an insoluble Cr(III) precipitate under aerobic and anaerobic conditions. CRB5 tolerated up to 520 mg of Cr(VI) liter?1 and reduced chromate in the presence of copper and arsenate. Under anaerobic conditions it also reduced Co(III) and U(VI), partially internalizing each metal. Metal precipitates were also found on the surface of the outer membrane and (sometimes) on a capsule. The results showed that chromate reduction by CRB5 was mediated by a soluble enzyme that was largely contained in the cytoplasm but also found outside of the cells. The crude reductase activity in the soluble fraction showed aKm of 23 mg liter?1 (437 μM) and a V max of 0.98 mg of Cr h?1 mg of protein?1 (317 nmol min?1 mg of protein?1). Minor membrane-associated Cr(VI) reduction under anaerobiosis may account for anaerobic reduction of chromate under nongrowth conditions with an organic electron donor present. Chromate reduction under both aerobic and anaerobic conditions may be a detoxification strategy for the bacterium which could be exploited to bioremediate chromate-contaminated or other toxic heavy metal-contaminated environments.
机译：从退役的木材保存场所分离出的假单胞菌（CRB5）在有氧和厌氧条件下将有毒的铬酸盐[Cr（VI）]还原为不溶的Cr（III）沉淀物。在铜和砷酸盐的存在下，CRB5最多可耐受520 mg Cr（VI）升？1，并还原铬酸盐。在厌氧条件下，它还还原了Co（III）和U（VI），部分内化了每种金属。在外膜的表面和（有时）在胶囊上也发现了金属沉淀。结果表明，CRB5的铬酸盐还原作用是由可溶性酶介导的，该酶主要包含在细胞质中，但也存在于细胞外。可溶性级分中的粗还原酶活性显示为23 Km·l·1（437μM）的Km和0.98 mg Cr·h·1 mg蛋白质·1（317 nmol·min·1 mg蛋白质·1）的V max。在厌氧条件下，与膜相关的Cr（VI）的少量还原可解释在无增长条件下存在有机电子给体的情况下铬酸盐的厌氧还原。在好氧和厌氧条件下还原铬酸盐可能是该细菌的解毒策略，可用于细菌生物修复铬酸盐污染或其他有毒重金属污染的环境。

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