Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

Julian R. Marchesi; Takuichi Sato; Andrew J. Weightman; Tracey A. Martin; John C. Fry; Sarah J. Hiom; William G. Wade

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Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

机译：设计和评估有用的细菌特异性PCR引物，该引物可扩增细菌16S rRNA的基因编码。

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We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

机译：我们报告的PCR引物63f和1387r的设计和评估，用于从细菌中扩增16S rRNA基因。用各种细菌和环境样品系统地测试了它们的特异性和功效。在生态和系统研究中，它们比16A rRNA基因扩增比目前更普遍使用的PCR扩增子更有用。

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《Applied and Environmental Microbiology》 |1998年第2期|共7页
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Julian R. Marchesi; Takuichi Sato; Andrew J. Weightman; Tracey A. Martin; John C. Fry; Sarah J. Hiom; William G. Wade;
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中图分类植物学;
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1. Factors affecting interpretation of restriction fragment length polymorphism (RFLP) patterns from PCR-amplified bacterial 16S rRNA genes: Operon number and primer mismatching [J] . Ramirez-Moreno S, Memdez-Alvarez S, Martinez-Alonso M, Current Microbiology: An International Journal . 2004,第4期

机译：影响从PCR扩增细菌16S rRNA基因限制性片段长度多态性（RFLP）模式解释的因素：操纵子数和引物错配
2. Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify eukaryotic rRNA genes [J] . MoriH., MaruyamaF., KatoH., DNA research: an international journal for rapid publication of reports on genes and genomes . 2014,第2期

机译：一种新颖的非简并通用引物组的设计和实验应用，该引物组扩增原核16S rRNA基因的可能性很小，不能扩增真核rRNA基因
3. Design and Experimental Application of a Novel Non-Degenerate Universal Primer Set that Amplifies Prokaryotic 16S rRNA Genes with a Low Possibility to Amplify Eukaryotic rRNA Genes [J] . Asao Fujiyama, Atsushi Toyoda, Ayumi Dozono, DNA research: an international journal for rapid publication of reports on genes and genomes . 2014,第2期

机译：一种新型的非简并通用引物组的设计和实验应用，该引物组可扩增具有低扩增真核rRNA基因的原核16S rRNA基因
4. Monitoring the microbiat community inhabiting a low-grade copper sulphide ore by Quantitative Real-Time PCR analysis of 16S rRNA genes [C] . F. Remonsellez, F. Galleguillos, S. Janse van Rensburg International Symposium on Biohydrometallurgy . 2007

机译：通过16S rRNA基因的定量实时PCR分析监测含有低级硫化铜矿石的微生物群落
5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

机译：使用PCR扩增的16S rRNA基因的变性梯度凝胶电泳，沿着盐梯度进行土壤群落分析。
6. Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA [O] . Julian R. Marchesi, Takuichi Sato, Andrew J. Weightman, 1998

机译：设计和评估有用的细菌特异性PCR引物该引物可扩增细菌16S rRNA的基因编码。
7. Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA [O] . Julian R. Marchesi, Takuichi Sato, Andrew J. Weightman, 1998

机译：用于扩增对细菌16s rRNA的基因的有用细菌特异性PCR引物的设计与评价

Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

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