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首页> 外文期刊>Applied and Environmental Microbiology >Characterization of Genes Involved in Biosynthesis of a Novel Antibiotic from Burkholderia cepacia BC11 and Their Role in Biological Control of Rhizoctonia solani
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Characterization of Genes Involved in Biosynthesis of a Novel Antibiotic from Burkholderia cepacia BC11 and Their Role in Biological Control of Rhizoctonia solani

机译：洋葱伯克霍尔德氏菌BC11新型抗生素生物合成相关基因的表征及其在茄根丝枯菌生物防治中的作用

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摘要

Genetic manipulation of fluorescent pseudomonads has provided major insight into their production of antifungal molecules and their role in biological control of plant disease. Burkholderia cepaciaalso produces antifungal activities, but its biological control activity is much less well characterized, in part due to difficulties in applying genetic tools. Here we report genetic and biochemical characterization of a soil isolate of B. cepacia relating to its production of an unusual antibiotic that is very active against a variety of soil fungi. Purification and preliminary structural analyses suggest that this antibiotic (called AFC-BC11) is a novel lipopeptide associated largely with the cell membrane. Analysis of conditions for optimal production of AFC-BC11 indicated stringent environmental regulation of its synthesis. Furthermore, we show that production of AFC-BC11 is largely responsible for the ability ofB. cepacia BC11 to effectively control the damping-off of cotton caused by the fungal pathogen Rhizoctonia solani in a gnotobiotic system. Using Tn5 mutagenesis, we identified, cloned, and characterized a region of the genome of strain BC11 that is required for production of this antifungal metabolite. DNA sequence analysis suggested that this region encodes proteins directly involved in the production of a nonribosomally synthesized lipopeptide.
机译：荧光假单胞菌的遗传操作已为他们的抗真菌分子生产及其在植物病害生物防治中的作用提供了重要见解。洋葱伯克霍尔德菌也产生抗真菌活性，但其生物防治活性的特征却差强人意，部分原因是应用遗传工具有困难。在这里，我们报告了洋葱头孢菌的土壤分离物的遗传和生化特征，这与它产生的一种对多种土壤真菌具有非常活性的非常规抗生素有关。纯化和初步结构分析表明，这种抗生素（称为AFC-BC11）是一种新型脂肽，主要与细胞膜相关。对AFC-BC11最佳生产条件的分析表明，其合成受到严格的环境监管。此外，我们表明，AFC-BC11的产生在很大程度上负责B的能力。 cepacia BC11可以有效控制真菌在病原菌系统中引起的病原体Rhisthonia solani。使用Tn5诱变，我们鉴定，克隆和鉴定了BC11菌株基因组中产生这种抗真菌代谢产物所需的区域。 DNA序列分析表明，该区域编码直接参与非核糖体合成脂肽生产的蛋白质。

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