Detection and Quantification of Dehalogenimonas and “Dehalococcoides” Populations via PCR-Based Protocols Targeting 16S rRNA Genes

Jun Yan; Brian A. Rash; Fred A. Rainey; William M. Moe

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Detection and Quantification of Dehalogenimonas and “Dehalococcoides” Populations via PCR-Based Protocols Targeting 16S rRNA Genes

机译：通过针对16S rRNA基因的基于PCR的协议检测和定量Dehalogenimonas和“ Dehalococcoides”种群。

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Members of the haloalkane dechlorinating genus Dehalogenimonas are distantly related to “Dehalococcoides” but share high homology in some variable regions of their 16S rRNA gene sequences. In this study, primers and PCR protocols intended to uniquely target Dehalococcoides were reevaluated, and primers and PCR protocols intended to uniquely target Dehalogenimonas were developed and tested. Use of the genus-specific primers revealed the presence of both bacterial groups in groundwater at a Louisiana Superfund site.

机译：卤代烷脱氯属Dehalogenimonas的成员与“ Dehalococcoides”关系密切，但在其16S rRNA基因序列的某些可变区具有高度同源性。在这项研究中，重新评估了旨在唯一靶向Dehalococcoides的引物和PCR方案，并开发并测试了旨在唯一靶向Dehalogenimonas的引物和PCR方案。属特异性引物的使用揭示了路易斯安那州超级基金所在地地下水中两个细菌群的存在。

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《Applied and Environmental Microbiology》 |2009年第23期|共5页
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Jun Yan; Brian A. Rash; Fred A. Rainey; William M. Moe;
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1. Reassessment of PCR primers targeting 16S rRNA genes of the organohalide-respiring genus Dehalogenimonas [J] . Chen Jie, Bowman Kimberly S., Rainey Fred A., Biodegradation . 2014,第5期

机译：靶向呼吸有机卤化物Dehalogenimonas的16S rRNA基因的PCR引物的重新评估
2. Genus- and Species-Specific PCR-Based Detection of Dairy Propionibacteria in Environmental Samples by Using Primers Targeted to the Genes Encoding 16S rRNA [J] . Franca Rossi, Sandra Torriani, Franco Dellaglio Applied and Environmental Microbiology . 1999,第9期

机译：通过使用针对16S rRNA编码基因的引物，基于属和种的PCR检测环境样品中的乳丙酸杆菌。
3. Specific Detection of Dehalococcoides Species by Fluorescence In Situ Hybridization with 16S rRNA-Targeted Oligonucleotide Probes [J] . Yanru Yang, Josef Zeyer Applied and Environmental Microbiology . 2003,第5期

机译：通过与16S rRNA靶向的寡核苷酸探针的荧光原位杂交特异性检测Dehalococcoides物种。
4. Identification of human fecal pollution: detection and quantification of the human specific HF183 Bacteroides 16S rRNA genetic marker with real-time PCR [C] . S. Seurinck, T. Defoirt, W. Verstraete, European Symposium on Environmental Biotechnology(ESEB 2004); 20040425-20040428; Oostende; BE . 2004

机译：鉴定人类粪便污染：实时PCR检测和定量人类特异性HF183拟杆菌16S rRNA遗传标记
5. Development of a 16S rRNA targeted probe to identify and characterize Lactobacillus populations [D] . Lawson, Melissa A. E. 2007

机译：16S rRNA靶向探针的开发，以鉴定和表征乳杆菌种群
6. Detection and Quantification of Dehalogenimonas and Dehalococcoides Populations via PCR-Based Protocols Targeting 16S rRNA Genes [O] . Jun Yan, Brian A. Rash, Fred A. Rainey, 2009

机译：通过针对16S rRNA基因的基于PCR的协议检测和定量Dehalogenimonas和 Dehalococcoides种群。
7. Detection and Quantification of Dehalogenimonas and “Dehalococcoides” Populations via PCR-Based Protocols Targeting 16S rRNA Genes▿ † [O] . Yan, Jun, Rash, Brian A., Rainey, Fred A., 2009

机译：通过针对16S rRNA基因的基于PCR的方案检测和定量Dehalogenimonas和“ Dehalococcoides”种群

Detection and Quantification of Dehalogenimonas and “Dehalococcoides” Populations via PCR-Based Protocols Targeting 16S rRNA Genes

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