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首页> 外文期刊>Applied and Environmental Microbiology >Complete Genomic Sequence of Bacteriophage φEcoM-GJ1, a Novel Phage That Has Myovirus Morphology and a Podovirus-Like RNA Polymerase
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Complete Genomic Sequence of Bacteriophage φEcoM-GJ1, a Novel Phage That Has Myovirus Morphology and a Podovirus-Like RNA Polymerase

机译：噬菌体φEcoM-GJ1的完整基因组序列，一种新型的噬菌体，具有肌病毒形态学和足病毒样RNA聚合酶

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The complete genome of φEcoM-GJ1, a lytic phage that attacks porcine enterotoxigenic Escherichia coli of serotype O149:H10:F4, was sequenced and analyzed. The morphology of the phage and the identity of the structural proteins were also determined. The genome consisted of 52,975 bp with a G+C content of 44% and was terminally redundant and circularly permuted. Seventy-five potential open reading frames (ORFs) were identified and annotated, but only 29 possessed homologs. The proteins of five ORFs showed homology with proteins of phages of the family Myoviridae, nine with proteins of phages of the family Podoviridae, and six with proteins of phages of the family Siphoviridae. ORF 1 encoded a T7-like single-subunit RNA polymerase and was preceded by a putative E. coli σ70-like promoter. Nine putative phage promoters were detected throughout the genome. The genome included a tRNA gene of 95 bp that had a putative 18-bp intron. The phage morphology was typical of phages of the family Myoviridae, with an icosahedral head, a neck, and a long contractile tail with tail fibers. The analysis shows that φEcoM-GJ1 is unique, having the morphology of the Myoviridae, a gene for RNA polymerase, which is characteristic of phages of the T7 group of the Podoviridae, and several genes that encode proteins with homology to proteins of phages of the family Siphoviridae.
机译：对φEcoM-GJ1（攻击O149：H10：F4型猪肠毒素大肠杆菌的裂解噬菌体）的完整基因组进行了测序和分析。还确定了噬菌体的形态和结构蛋白的身份。该基因组由52,975 bp组成，G + C含量为44％，末端冗余且循环排列。鉴定并注释了75个潜在的开放阅读框（ORF），但只有29个具有同源物。五个ORF的蛋白质与Myoviridae家族的噬菌体蛋白同源，九个与Podoviridae家族的噬菌体蛋白同源，六个与Siphoviridae家族的噬菌体蛋白同源。 ORF 1编码一个T7样单亚基RNA聚合酶，并在假定的大肠杆菌σ70样启动子之前。在整个基因组中检测到九个假定的噬菌体启动子。基因组包括一个95 bp的tRNA基因，该基因具有一个18 bp的内含子。噬菌体的形态是Myoviridae家族的典型噬菌体，具有二十面体的头部，颈部和一条长尾巴的尾巴，尾巴纤维。分析表明，φEcoM-GJ1具有独特性，具有Myoviridae的形态，RNA聚合酶基因（其是Podoviridae的T7组噬菌体的特征）以及几个与该噬菌体的噬菌体蛋白同源的蛋白编码基因。家庭Siphoviridae。

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