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首页> 外文期刊>Applied and Environmental Microbiology >Gene Expression Analysis of Copper Tolerance and Wood Decay in the Brown Rot Fungus Fibroporia radiculosa
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Gene Expression Analysis of Copper Tolerance and Wood Decay in the Brown Rot Fungus Fibroporia radiculosa

机译：褐腐真菌Fibroporia radiculosa铜耐性和木材腐烂的基因表达分析

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High-throughput transcriptomics was used to identify Fibroporia radiculosa genes that were differentially regulated during colonization of wood treated with a copper-based preservative. The transcriptome was profiled at two time points while the fungus was growing on wood treated with micronized copper quat (MCQ). A total of 917 transcripts were differentially expressed. Fifty-eight of these genes were more highly expressed when the MCQ was protecting the wood from strength loss and had putative functions related to oxalate production/degradation, laccase activity, quinone biosynthesis, pectin degradation, ATP production, cytochrome P450 activity, signal transduction, and transcriptional regulation. Sixty-one genes were more highly expressed when the MCQ lost its effectiveness (>50% strength loss) and had functions related to oxalate degradation; cytochrome P450 activity; H2O2 production and degradation; degradation of cellulose, hemicellulose, and pectin; hexose transport; membrane glycerophospholipid metabolism; and cell wall chemistry. Ten of these differentially regulated genes were quantified by reverse transcriptase PCR for a more in-depth study (4 time points on wood with or without MCQ treatment). Our results showed that MCQ induced higher than normal levels of expression for four genes (putative annotations for isocitrate lyase, glyoxylate dehydrogenase, laccase, and oxalate decarboxylase 1), while four other genes (putative annotations for oxalate decarboxylase 2, aryl alcohol oxidase, glycoside hydrolase 5, and glycoside hydrolase 10) were repressed. The significance of these results is that we have identified several genes that appear to be coregulated, with putative functions related to copper tolerance and/or wood decay.
机译：高通量转录组学被用来鉴定在用铜基防腐剂处理的木材定居过程中受到不同调节的Radi Fiporporia radiculosa基因。在真菌在经过微粉化铜季铵盐（MCQ）处理的木材上生长时，在两个时间点对转录组进行了分析。总共有917个转录物被差异表达。当MCQ保护木材免受强度损失时，这些基因中的58个基因表达更高，并具有与草酸盐生成/降解，漆酶活性，醌生物合成，果胶降解，ATP生成，细胞色素P450活性，信号转导，和转录调控。当MCQ失去效力（> 50％的强度损失）并具有与草酸盐降解相关的功能时，有61个基因的表达更高。细胞色素P450活性; H2O2的产生和降解;纤维素，半纤维素和果胶的降解;己糖运输膜糖脂代谢和细胞壁化学。通过逆转录酶PCR定量这些差异调节基因中的10个，用于更深入的研究（在有或没有MCQ处理的情况下，在木材上有4个时间点）。我们的结果表明，MCQ诱导四个基因（异柠檬酸裂合酶，乙醛酸脱氢酶，漆酶和草酸脱羧酶1的潜在注释）的表达水平高于正常水平，而其他四个基因（草酸脱羧酶2，芳醇氧化酶，糖苷的潜在注释）水解酶5和糖苷水解酶10）被抑制。这些结果的意义在于，我们已经鉴定出几个似乎被调控的基因，其推定功能与铜耐性和/或木材腐烂有关。

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