• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >LuxR- and LuxI-Type Quorum-Sensing Circuits Are Prevalent in Members of the Populus deltoides Microbiome
【24h】

LuxR- and LuxI-Type Quorum-Sensing Circuits Are Prevalent in Members of the Populus deltoides Microbiome

机译：LuxR型和LuxI型群体感应电路在Populus deltoides微生物组成员中很普遍。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We are interested in the root microbiome of the fast-growing Eastern cottonwood tree, Populus deltoides. There is a large bank of bacterial isolates from P. deltoides, and there are 44 draft genomes of bacterial endophyte and rhizosphere isolates. As a first step in efforts to understand the roles of bacterial communication and plant-bacterial signaling in P. deltoides, we focused on the prevalence of acyl-homoserine lactone (AHL) quorum-sensing-signal production and reception in members of the P. deltoides microbiome. We screened 129 bacterial isolates for AHL production using a broad-spectrum bioassay that responds to many but not all AHLs, and we queried the available genome sequences of microbiome isolates for homologs of AHL synthase and receptor genes. AHL signal production was detected in 40% of 129 strains tested. Positive isolates included members of the Alpha-, Beta-, and Gammaproteobacteria. Members of the luxI family of AHL synthases were identified in 18 of 39 proteobacterial genomes, including genomes of some isolates that tested negative in the bioassay. Members of the luxR family of transcription factors, which includes AHL-responsive factors, were more abundant than luxI homologs. There were 72 in the 39 proteobacterial genomes. Some of the luxR homologs appear to be members of a subfamily of LuxRs that respond to as-yet-unknown plant signals rather than bacterial AHLs. Apparently, there is a substantial capacity for AHL cell-to-cell communication in proteobacteria of the P. deltoides microbiota, and there are also Proteobacteria with LuxR homologs of the type hypothesized to respond to plant signals or cues.
机译：我们对快速生长的东部三角叶杨树Populus deltoides的根微生物组感兴趣。有大量来自三角假单胞菌的细菌分离株，并且细菌内生菌和根际分离株有44个草图基因组。作为努力了解三角洲假单胞菌中细菌传播和植物细菌信号传导作用的第一步，我们重点研究了P-成员中酰基高丝氨酸内酯（AHL）群体感应信号产生和接受的普遍性。三角洲微生物组。我们使用了对许多但不是全部AHL都有反应的广谱生物测定法，筛选了129株分离出AHL的细菌，并针对AHL合酶和受体基因的同源性查询了微生物组分离物的可用基因组序列。在测试的129个菌株中有40％检测到AHL信号产生。阳性分离株包括Alpha，Beta和Gammaproteobacteria的成员。在39个变形杆菌基因组中的18个中鉴定了luxI AHL合酶家族的成员，包括在生物测定中测试为阴性的一些分离株的基因组。包括AHL响应因子在内的luxR转录因子家族成员比luxI同源物丰富。 39个蛋白酶基因组中有72个。一些luxR同源物似乎是LuxR的一个亚家族的成员，该亚家族对迄今未知的植物信号而不是细菌AHL作出反应。显然，在三角假单胞菌菌群的细菌中，AHL与细胞之间的交流具有很大的能力，并且还存在被认为对植物信号或线索有反应的具有LuxR同源物的细菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号