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Survival of Clostridium botulinum Spores

机译:肉毒梭菌孢子的存活

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Radiation survival curves of spores of Clostridium botulinum strain 33A exhibited an exponential reduction which accounted for most of the population, followed by a “tail” comprising a very small residual number [7 to 0.7 spore(s) per ml] which resisted death in the range between 3.0 and 9.0 Mrad dose levels. The “tail” was not caused by protective spore substances released into the suspensions during irradiation, by the presence of accumulated radiation “inactivated” spores, or by heat shock of pre-irradiated spores. The theoretical number of spore targets which must be inactivated by irradiation was estimated both by a graphical and by a computation method to be about 80, and the D value was calculated to be 0.295 and 0.396 Mrad, respectively, in buffer and in pork pea broth.
机译:肉毒梭菌33A菌株的孢子的放射存活曲线显示出指数减少,占总人口的大部分,其后是“尾巴”,其残留量很小(每毫升7到0.7个孢子),可以抵抗死亡。剂量范围在3.0至9.0 Mrad之间。 “尾巴”不是由辐射过程中释放到悬浮液中的保护性孢子物质引起的,不是由于辐射“灭活”的累积孢子的存在,也不是预辐照的孢子的热激引起的。用图解法和计算方法估算必须通过辐照灭活的孢子靶的理论数为约80,在缓冲液和豌豆汤中D值分别为0.295和0.396 Mrad。 。

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