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首页> 外文期刊>Applied and Environmental Microbiology >Arthrobacter Strain VAI-A Utilizes Acyl-Homoserine Lactone Inactivation Products and Stimulates Quorum Signal Biodegradation by Variovorax paradoxus
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Arthrobacter Strain VAI-A Utilizes Acyl-Homoserine Lactone Inactivation Products and Stimulates Quorum Signal Biodegradation by Variovorax paradoxus

机译：关节杆菌菌株VAI-A利用酰基同型内酯内酯灭活产物并刺激Variovorax paradoxus的群体信号生物降解。

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Many Proteobacteria produce acyl-homoserine lactones (acyl-HSLs) and employ them as dedicated cell-to-cell signals in a process known as quorum sensing. Previously, Variovorax paradoxus VAI-C was shown to utilize diverse acyl-HSLs as sole sources of energy and nitrogen. We describe here the properties of a second isolate, Arthrobacter strain VAI-A, obtained from the same enrichment culture that yielded V. paradoxus VAI-C. Although strain VAI-A grew rapidly and exponentially on a number of substrates, it grew only slowly and aberrantly (i.e., linearly) in media amended with oxohexanoyl-HSL as the sole energy source. Increasing the culture pH markedly improved the growth rate in media containing this substrate but did not abolish the aberrant kinetics. The observed growth was remarkably similar to the known kinetics of the pH-influenced half-life of acyl-HSLs, which decay chemically to yield the corresponding acyl-homoserines. Strain VAI-A grew rapidly and exponentially when provided with an acyl-homoserine as the sole energy or nitrogen source. The isolate was also able to utilize HSL as a sole source of nitrogen but not as energy for growth. V. paradoxus, known to release HSL as a product of quorum signal degradation, was examined for the ability to support the growth of Arthrobacter strain VAI-A in defined cocultures. It did. Moreover, the acyl-HSL-dependent growth rate and yield of the coculture were dramatically superior to those of the monocultures. This suggested that the original coenrichment of these two organisms from the same soil sample was not coincidental and that consortia may play a role in quorum signal turnover and mineralization. The fact that Arthrobacter strain VAI-A utilizes the two known nitrogenous degradation products of acyl-HSLs, acyl-homoserine and HSL, begins to explain why none of the three compounds are known to accumulate in the environment.
机译：许多ro> Proteobacteria 会产生酰基-高丝氨酸内酯（酰基-HSL），并在称为群体感应的过程中将其用作专用的细胞间信号。以前， Variovorax paradoxus VAI-C被证明利用多种酰基-HSL作为唯一的能量和氮源。我们在这里描述了第二种分离株 Arthrobacter 菌株VAI-A的特性，该菌株是从产生 V的相同富集培养物中获得的。悖论VAI-C。尽管菌株VAI-A在许多底物上迅速且呈指数增长，但在以氧己酰-HSL作为唯一能源改良的培养基中，它仅缓慢而异常地（即线性地）生长。提高培养液的pH值可显着提高含有该底物的培养基的生长速率，但不会消除异常动力学。观察到的生长与pH影响的酰基-HSLs半衰期的已知动力学显着相似，后者化学降解后会生成相应的酰基-高丝氨酸。当提供酰基高丝氨酸作为唯一能源或氮源时，菌株VAI-A迅速且呈指数增长。该分离株还能够利用HSL作为唯一的氮源，但不能作为生长的能量。 V。在已知的共培养物中，对已知会释放定语信号降解产物HSL的悖论进行了研究，以支持 Arthrobacter 菌株VAI-A的生长能力。它做了。此外，共培养的酰基-HSL依赖性生长速率和产量显着优于单培养。这表明来自同一土壤样品的这两种生物最初的共富集不是偶然的，并且财团可能在群体信号转换和矿化中发挥作用。炭疽杆菌菌株VAI-A利用了酰基-HSL的两种已知的含氮降解产物，即酰基高丝氨酸和HSL，这一事实开始解释了为什么三种化合物都不在环境中积累。

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