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首页> 外文期刊>Applied and Environmental Microbiology >Radioisotopic, Culture-Based, and Oligonucleotide Microchip Analyses of Thermophilic Microbial Communities in a Continental High-Temperature Petroleum Reservoir
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Radioisotopic, Culture-Based, and Oligonucleotide Microchip Analyses of Thermophilic Microbial Communities in a Continental High-Temperature Petroleum Reservoir

机译：放射性同位素，基于文化和寡核苷酸芯片分析大陆高温石油库中的嗜热微生物群落。

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Activity measurements by radioisotopic methods and cultural and molecular approaches were used in parallel to investigate the microbial biodiversity and its physiological potential in formation waters of the Samotlor high-temperature oil reservoir (Western Siberia, Russia). Sulfate reduction with rates not exceeding 20 nmol of H2S liter?1 day?1 occurred at 60 and 80°C. In upper horizons (AB, A, and B), methanogenesis (lithotrophic and/or acetoclastic) was detected only in wells in which sulfate reduction did not occur. In some of the wells from deeper (J) horizons, high-temperature sulfate reduction and methanogenesis occurred simultaneously, the rate of lithotrophic methanogenesis exceeding 80 nmol of CH4 liter?1 day?1. Enrichment cultures indicated the presence of diverse physiological groups representing aerobic and anaerobic thermophiles and hyperthermophiles; fermentative organotrophs were predominant. Phylogenetic analyses of 15 isolates identified representatives of the genera Thermotoga, Thermoanaerobacter, Geobacillus, Petrotoga, Thermosipho, and Thermococcus, the latter four being represented by new species. Except for Thermosipho, the isolates were members of genera recovered earlier from similar habitats. DNA obtained from three samples was hybridized with a set of oligonucleotide probes targeting selected microbial groups encompassing key genera of thermophilic bacteria and archaea. Oligonucleotide microchip analyses confirmed the cultural data but also revealed the presence of several groups of microorganisms that escaped cultivation, among them representatives of the Aquificales/Desulfurobacterium-Thermovibrio cluster and of the genera Desulfurococcus and Thermus, up to now unknown in this habitat. The unexpected presence of these organisms suggests that their distribution may be much wider than suspected.
机译：平行使用放射性同位素方法，文化和分子方法进行活性测量，以研究Samotlor高温油藏（俄罗斯西伯利亚）地层水中的微生物多样性及其生理潜力。在60和80°C时，硫酸盐的还原速率不超过H2S升？1天？1的20 nmol。在较高层（AB，A和B），仅在未发生硫酸盐还原的井中检测到甲烷生成（溶营养和/或乙酰碎屑）。在（J）较深层的一些井中，高温硫酸盐还原和甲烷生成同时发生，岩石营养的甲烷生成速率超过CH4升？1天？1的80 nmol。富集培养表明存在着不同的生理群体，分别代表需氧和厌氧嗜热菌和嗜热菌。发酵性有机营养是主要的。对15个分离株的系统进化分析确定了Thermotoga，Thermanoaerobacter，Geobacillus，Petrotoga，Thermosipho和Thermococcus属的代表，后四个以新物种为代表。除Thermosipho外，这些分离株是较早从相似栖息地恢复的属的成员。从三个样品中获得的DNA与一组寡核苷酸探针杂交，该探针针对包括嗜热细菌和古细菌关键属的选定微生物群。寡核苷酸微芯片分析证实了文化数据，但也揭示了几组逃脱栽培的微生物的存在，其中包括无水杆菌/脱硫杆菌-嗜热菌群以及脱硫球菌和嗜热菌属的代表，迄今为止在该栖息地尚不知道。这些生物的出乎意料的存在表明它们的分布可能比怀疑的要广泛得多。

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