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首页> 外文期刊>Applied and Environmental Microbiology >Initiation of Protein Synthesis by a Labeled Derivative of the Lactobacillus casei DN-114 001 Strain during Transit from the Stomach to the Cecum in Mice Harboring Human Microbiota
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Initiation of Protein Synthesis by a Labeled Derivative of the Lactobacillus casei DN-114 001 Strain during Transit from the Stomach to the Cecum in Mice Harboring Human Microbiota

机译：在带有人类微生物群的小鼠从胃到盲肠的转移过程中，干酪乳杆菌DN-114 001菌株的标记衍生物引发蛋白质合成。

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Although studies on the survival of bacteria in the digestive tract have been reported in the literature, little data are available on the physiological adaptation of probiotics to the digestive environment. In previous work, a transcriptional fusion system (i.e., luciferase genes under the control of a deregulated promoter) was used to demonstrate that a derivative of the Lactobacillus casei DN-114 001 strain, ingested in a fermented milk and thus exhibiting initially a very weak metabolic activity, synthesized proteins de novo after its transit in the digestive tract of mice harboring human microbiota (known as human-microbiota-associated mice). With the same genetic system and animal model, we here investigate for the first time the ability of L. casei to reinitiate synthesis in the different digestive tract compartments. In this study, most ingested L. casei cells transited from the stomach to the duodenum-jejunum within 1 h postingestion. No luciferase activity was observed in these digestive tract compartments after the first hour. At later times, the bulk of bacteria had transited to the ileum and the cecum. Luciferase synthesis was detected between 1.5 and 2.0 h postingestion at the ileal level and from 1.5 h to at least 6.0 h postingestion in the cecum, where the activity remained at a maximum level. These results demonstrate that ingested L. casei (derivative of the DN-114 001 strain) administered via a fermented milk has already reinitiated protein synthesis when it reaches the ileal and cecal compartments.
机译：尽管文献中已经报道了有关细菌在消化道中存活情况的研究，但是关于益生菌对消化环境的生理适应性的数据很少。在以前的工作中，转录融合系统（即荧光素酶基因在失活的启动子控制下）被用来证明干酪乳杆菌DN-114 001菌株的衍生物被摄入发酵乳中，因此最初表现出非常弱的特性。代谢活动是指合成蛋白质从其在具有人类微生物群的小鼠（称为人类微生物群相关的小鼠）的消化道中迁移后重新产生。使用相同的遗传系统和动物模型，我们在这里首次调查干酪乳杆菌在不同消化道区室中重新启动合成的能力。在这项研究中，大多数摄入的干酪乳杆菌细胞在分泌后1小时内从胃部转移到了十二指肠空肠。在第一个小时后，在这些消化道区室中未观察到萤光素酶活性。后来，大部分细菌已经转移到回肠和盲肠。在回肠水平的1.5至2.0小时后检测盲肠中的荧光素酶合成，在盲肠中的1.5h至至少6.0小时后检测盲肠，其中活性保持在最高水平。这些结果表明，经发酵的乳汁摄入的干酪乳杆菌（DN-114 001菌株的衍生物）在到达回肠和盲肠区室时已经重新开始蛋白质合成。

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