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Disruption of the Subtilase Gene, albin1, in Ophiostoma piliferum

机译：颠末的Ophiostoma piliferum中枯草蛋白酶基因albin1的破坏。

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Wood sapstaining fungi produce multiple proteases that break down wood protein. Three groups of subtilases have been identified in sapstaining fungi; however, it is not known if these groups have distinct physiological roles (B. Hoffman and C. Breuil, Curr. Genet. 41:168-175, 2002). In this work we examined the role of the subtilase Albin1 from Ophiostoma piliferum. Reamplification of cDNA ends PCR was used to obtain the albin1 gene sequence. The encoded subtilase is probably extracellular and involved in nutrient acquisition. This gene was disrupted with an Agrobacterium tumefaciens-mediated transformation system. Two of the disruptants obtained had significantly lower levels of proteolytic activity, slower growth in bovine serum albumin, and significantly reduced growth on wood. Thus, albin1 plays an important role in O. piliferum's ability to acquire nitrogen from wood proteins.
机译：木质花木真菌产生多种分解木质蛋白的蛋白酶。枯萎真菌中已鉴定出三组枯草杆菌酶;然而，尚不清楚这些基团是否具有独特的生理作用（B.Hoffman和C.Breuil，Curr.Genet.41：168-175，2002）。在这项工作中，我们检查了来自Ophiostoma piliferum的枯草蛋白酶Albin1的作用。 cDNA末端的再扩增PCR用于获得albin1基因序列。编码的枯草蛋白酶可能在细胞外，并参与营养获取。该基因被根癌农杆菌介导的转化系统破坏。获得的两种破坏剂的蛋白水解活性水平明显较低，牛血清白蛋白的生长速度较慢，并且在木材上的生长速度明显降低。因此，albin1在皮氏酵母从木材蛋白质中获取氮的能力中起着重要作用。

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