• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Analysis of the gyrA Gene of Clinical Yersinia ruckeri Isolates with Reduced Susceptibility to Quinolones
【24h】

Analysis of the gyrA Gene of Clinical Yersinia ruckeri Isolates with Reduced Susceptibility to Quinolones

机译：减少对喹诺酮类药物的临床小肠耶尔森氏菌菌株的gyrA基因的分析

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Antimicrobial susceptibility of seven clinical strains of Yersinia ruckeri representative of those isolated between 1994 and 2002 from a fish farm with endemic enteric redmouth disease was studied. All isolates displayed indistinguishable pulsed-field gel electrophoresis restriction patterns, indicating that they represented a single strain. However, considering both inhibition zone diameters (IZD) and MICs, the isolates recovered in 2001-2002 formed a separate cluster with lower levels of susceptibility to all the quinolones tested, especially nalidixic acid (NA) and oxolinic acid (OA), compared with the isolates recovered between 1994 and 1998. Analysis of the PCR product of the quinolone resistance-determining region of the gyrA gene from clinical isolates of Y. ruckeri with reduced susceptibility to OA and NA revealed a single amino acid substitution, Ser-83 to Arg-83 (Escherichia coli numbering). Identical substitution was observed in induced OA-resistant mutant strains, which displayed IZD and MICs of quinolones similar to those of the clinical isolates of Y. ruckeri with reduced susceptibility to these antimicrobial agents. These data indicate in that for Y. ruckeri, the substitution of Ser by Arg at position 83 of the gyrA gene is associated with reduced susceptibility to quinolones.
机译：研究了1994年至2002年间从地方性肠红嘴病的一个养鱼场分离出的七株临床耶尔森氏菌的耐药性。所有分离株均显示出无法区分的脉冲场凝胶电泳限制性图谱，表明它们代表单个菌株。然而，考虑到抑制区直径（IZD）和MIC，在2001-2002年回收的分离株形成了一个单独的簇，对所有测试的喹诺酮类药物，特别是萘啶酸（NA）和草酸（OA）的敏感性较低。分离株在1994年至1998年之间恢复。对来自y.ruckeri的临床分离株的gyolA抗性决定区域的喹诺酮抗性区域的PCR产物的分析表明，其对OA和NA的敏感性降低，显示了一个单一的氨基酸取代，Ser-83取代了Arg -83（大肠杆菌编号）。在诱导的OA耐药突变株中观察到相同的取代，该菌株显示出喹诺酮类药物的IZD和MIC与Ruckeri的临床分离株相似，并且对这些抗菌剂的敏感性降低。这些数据表明，对于Y.ruckeri，在gyrA基因的83位被Arg取代Ser与降低对喹诺酮的敏感性有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号