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首页> 外文期刊>Applied and Environmental Microbiology >Development of a Cell Surface Display System in a Magnetotactic Bacterium, “Magnetospirillum magneticum” AMB-1
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Development of a Cell Surface Display System in a Magnetotactic Bacterium, “Magnetospirillum magneticum” AMB-1

机译：趋磁细菌“ Magnetospirillum magneticum” AMB-1中细胞表面显示系统的开发

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摘要

Bacterial cell surface display is a widely used technology for bioadsorption and for the development of a variety of screening systems. Magnetotactic bacteria are unique species of bacteria due to the presence of magnetic nanoparticles within them. These intracellular, nanosized (50 to 100 nm) magnetic nanoparticles enable the cells to migrate and be manipulated by magnetic force. In this work, using this unique characteristic and based on whole-genomic and comprehensive proteomic analyses of these bacteria, a cell surface display system has been developed by expressing hexahistidine residues within the outer coiled loop of the membrane-specific protein (Msp1) of the “Magnetospirillum magneticum” (proposed name) AMB-1 bacterium. The optimal display site of the hexahistidine residues was successfully identified via secondary structure prediction, immunofluorescence microscopy, and heavy metal binding assay. The established AMB-1 transformant showed high immunofluorescence response, high Cd2+ binding, and high recovery efficiency in comparison to those of the negative control when manipulated by magnetic force.
机译：细菌细胞表面展示是一种广泛用于生物吸附和开发各种筛选系统的技术。趋磁细菌是细菌的独特物种，因为其中存在磁性纳米颗粒。这些细胞内纳米大小（50至100 nm）的磁性纳米粒子使细胞能够迁移并受到磁力的操纵。在这项工作中，利用这种独特的特征，并基于对这些细菌的全基因组和全面的蛋白质组学分析，已经开发了一种细胞表面展示系统，该系统可以通过在细菌膜特异性蛋白（Msp1）的外螺旋环内表达六组氨酸残基来开发细胞表面展示系统。 “ Magnetospirillum magneticum”（建议名称）AMB-1细菌。通过二级结构预测，免疫荧光显微镜和重金属结合测定法成功地确定了六组氨酸残基的最佳展示位点。与通过磁力操作的阴性对照相比，已建立的AMB-1转化体显示出较高的免疫荧光反应，较高的Cd2 +结合力和较高的回收效率。

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