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Anaerobic Simulated Mixed Culture System

机译：厌氧模拟混合培养系统

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A compartmented, autoclavable culture vessel has been developed for the purpose of studying interactive associations of microorganisms which are essential to the anaerobic decomposition of sewage sludge. The unit employs sterile filter membranes to subdivide the interior culture space into individual compartments. Bacteria cultured in one compartment are denied access to adjacent compartments, even though rapid interchange of nutrients and metabolic waste products occurs throughout the unit. The obligate methane-forming anaerobe, Methanobacillus omelianskii has been successfully grown and concentrated in this system by use of a synthetic medium reduced with sodium sulfide. The feasibility of using this system to study microbial interactions was, in part, demonstrated by growing M. omelianskii in a thoroughly aerated medium which had been biologically reduced by Escherichia coli prior to inoculation with the anaerobe. Under this condition of simulated mixed culture growth, the cell yield of both microorganisms, as well as specific metabolic activities ascribed to each organism, was readily monitored.
机译：为了研究微生物污泥厌氧分解必不可少的微生物的相互作用，开发了一种可高压灭菌的隔间培养皿。该单元采用无菌滤膜将内部培养空间细分为各个隔室。即使在整个单元中发生营养物和代谢废物的快速交换，在一个隔间中培养的细菌也无法进入相邻的隔间。通过使用经硫化钠还原的合成培养基，专性甲烷形成厌氧菌厌氧甲烷菌已成功生长并浓缩在该系统中。使用该系统研究微生物相互作用的可行性在一定程度上通过在完全曝气的培养基中生长omelianskii进行了证明，该培养基在被厌氧菌接种之前已被大肠杆菌生物还原。在模拟混合培养物生长的这种条件下，可以轻松监测两种微生物的细胞产量以及归因于每种生物的特定代谢活性。

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