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Kinetic Studies on the Destructive Action of Oxygen on Lyophilized Serratia marcescens

机译：氧对冻干粘质沙雷氏菌的破坏作用的动力学研究

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Dried Serratia marcescens (ATTC strain 14041) cells were exposed to various partial pressures of oxygen and nitrogen. The colony-forming ability of the organisms was rapidly destroyed during exposure to oxygen but was unimpaired by exposure to purified nitrogen. The degree of inactivation depended upon temperature, time, and the partial pressure of oxygen, regardless of whether pure oxygen or dry air was used. The inactivation by oxygen followed the expression -1nN/N0 = k[O2]1/3t1/2, where N0 and N are the number of viable organisms before and after exposure respectively, [O2] is oxygen concentration, t is time, and k is the rate constant. At 25 C, k was 276 ± 36 moles-1/3 cc1/2 hr-1/3 for oxygen pressures between 5.5 and 258 torr. In the temperature range between -78 and 40 C, the rate constant may be expressed as k = 105.95±04.2 exp[(-430 ± 26) cal/RT] moles-1/3 cc1/3 hr-1/2.
机译：将干燥的粘质沙雷氏菌（ATTC菌株14041）细胞暴露于氧气和氮气的各种分压下。生物体的集落形成能力在暴露于氧气中迅速被破坏，但暴露于纯净的氮气并没有受到损害。灭活的程度取决于温度，时间和氧气的分压，而不管使用的是纯氧气还是干燥空气。被氧气灭活的表达式为-1nN / N0 = k [O2] 1 / 3t1 / 2，其中N0和N分别是暴露之前和之后的活菌数量，[O2]是氧气浓度，t是时间，并且k是速率常数。在25℃下，氧气压力在5.5至258托之间时，k为276±36摩尔-1/3 cc1 / 2 hr-1 / 3。在-78至40℃的温度范围内，速率常数可以表示为k ＝ 105.95±04.2exp [（-430±26）cal / RT]摩尔-1 / 3cc1 / 3hr-1 / 2。

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