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首页> 外文期刊>Applied Microbiology >Identification of Isolates of Clostridium perfringens Types C and D by Agglutination and Fluorescent-Antibody Methods
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Identification of Isolates of Clostridium perfringens Types C and D by Agglutination and Fluorescent-Antibody Methods

机译：凝集和荧光抗体方法鉴定产气荚膜梭状芽孢杆菌C型和D型分离株

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Agglutination and fluorescent-antibody methods were employed for screening Clostridium perfringens types C and D from 393 isolates of this organism. All of 50 strains which were isolated in Japan and were agglutinable with an antiserum prepared against a stock strain of type C no. 3182 toxigenically belonged to type C, but the antiserum showed no cross-agglutination with any of type C strains isolated in Denmark. All of the latter strains, however, were agglutinated by an antiserum prepared against a Danish strain, CWC11. Of 64 strains, showing heat-labile agglutinability by type D antiserum L9, 22 strains were toxigenically identified as type D strains which can be divided into three groups by the heat-stable antigens; no strains which were L-agglutination-positive but O-agglutination-negative were epislon-toxigenic. All of 13 strains, the heat-stable antigen of which was agglutinable by a type D antiserum VX81, were toxigenically type D strains. The results of fluorescent-antibody tests were almost in agreement with those of agglutination test with type C strains and completely with those of the O-agglutination test with type D strains. No beta-, epsilon- or delta-toxigenicity could be demonstrated in strains which were not agglutinated by our test sera for types C and D strains. Further examination of cultural properties of Japanese and Danish type C strains revealed that the two groups were considerably different in urease production, capsule formation, and delta- and alpha-toxigenicities.
机译：使用凝集和荧光抗体方法从该生物的393个分离物中筛选产气荚膜梭状芽胞杆菌C型和D型。在日本分离的所有50株菌株均可用针对C型C型原种菌株制备的抗血清进行凝集。 3182毒理学上属于C型，但抗血清与丹麦分离出的任何C型菌株均无交叉凝集。但是，所有后一种菌株都被针对丹麦菌株CWC11制备的抗血清凝集。在64种表现出D型抗血清L9热不稳定凝集性的菌株中，有22种被毒理学鉴定为D型菌株，可以通过热稳定抗原分为三类。没有L-凝集阳性但O-凝集阴性的菌株是依斯康毒原性的。 13种菌株的热稳定性抗原可被D型抗血清VX81凝集，均为毒理性D型菌株。荧光抗体试验的结果几乎与C型菌株的凝集试验结果一致，而与D型菌株的O凝集试验结果完全一致。在我们的C型和D型菌株的测试血清未凝集的菌株中，没有证明有β，ε或δ毒性。进一步检查日本和丹麦C型菌株的文化特性后发现，这两组在脲酶生产，胶囊形成以及δ和α毒性方面有很大不同。

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