Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa

Julia Giebler; Lukas Y. Wick; Michael Schloter; Hauke Harms; Antonis Chatzinotas

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Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa

机译：评估alkB终端限制片段和序列类型分配给不同的细菌类群

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Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

机译：序列和末端限制性片段长度多态性（T-RFLP）分析表明，土壤细菌分离物中有多个alkB基因拷贝/细胞，并且在各个系统发生群之间遗传迁移性明显较高。由于基于16S rRNA和alkB基因序列的系统树高度不一致，因此通过alkB末端限制性片段（T-RFs）和序列鉴定烷烃降解物的方法存在很大偏见。

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《Applied and Environmental Microbiology》 |2013年第9期|共4页
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Julia Giebler; Lukas Y. Wick; Michael Schloter; Hauke Harms; Antonis Chatzinotas;
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1. Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa [J] . Julia Giebler, Lukas Y. Wick, Michael Schloter, Applied Microbiology . 2013,第9期

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5. Qualitative assessments and computational techniques for the studies of microbial diversity based on terminal restriction fragment length polymorphism (T-RFLP) of 16S and 18S rRNA gene sequences [D] . Shyu, Conrad. 2006

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6. Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa [O] . Julia Giebler, Lukas Y. Wick, Michael Schloter, 2013

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7. Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA Gene Sequences [O] . I-Nung Huang, Yuri Sato, Mitsuo Sakamoto, 2014

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Evaluating the Assignment of alkB Terminal Restriction Fragments and Sequence Types to Distinct Bacterial Taxa

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