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Comparison of Fluorescent Antibody with Cultural Technique for Isolation of Enteropathogenic Escherichia coli from Swine

机译：荧光抗体与培养技术分离猪肠道致病性大肠杆菌的比较

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In an investigation of hogs as possible reservoirs of human strains of enteropathogenic Escherichia coli (EEC), 92 six-month-old grain- and garbage-fed hogs were examined on the farm and again at the packing plant. Of the 331 specimens obtained by swabbing the rectum, cecum, and edible meat carcass of these hogs, 125 were presumptively positive for EEC when screened by the fluorescent-antibody (FA) technique. These “presumptive positive” specimens then underwent extensive bacteriological examination and complete serological typing. The FA technique proved to be an easier, simpler, and more economical procedure than culture when a large number of specimens were examined for possible EEC serogroups. It was found especially valuable for identification of multiple serogroups of EEC within a single specimen. It also appeared to be more sensitive than cultural examination, since results were not dependent on the presence of large numbers of organisms in the specimen, or even on their viability. However, the FA technique was found to be less specific than culture because of cross-reactivity with antigenically related Enterobacteriaceae when fluorescein-labeled antisera were used. Therefore, any specimen found positive on FA examination should be considered as presumptive positive until confirmed by bacteriological examination and complete serological study.
机译：在对猪作可能的人类病原性大肠杆菌（EEC）菌株库进行调查中，对农场和包装厂的92只6个月大的谷物和垃圾喂养猪进行了检查。通过擦拭这些猪的直肠，盲肠和食用肉car体获得的331个样本中，当通过荧光抗体（FA）技术进行筛选时，假定有125个样本的EEC呈阳性。然后对这些“推定阳性”的标本进行广泛的细菌学检查和完整的血清学分型。当检查大量标本中可能存在的EEC血清群时，与培养相比，FA技术被证明是一种更容易，更简单，更经济的方法。发现它对于鉴定单个样本中的多个EEC血清群特别有价值。它似乎也比文化检查更敏感，因为结果不取决于标本中是否存在大量生物，甚至不取决于它们的生存力。然而，由于使用荧光素标记的抗血清时，与抗原相关肠杆菌科的交叉反应性，发现FA技术的特异性不如培养。因此，任何在FA检查中发现阳性的标本应视为推定阳性，直到通过细菌学检查和完整的血清学研究证实为止。

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