Quantitative Analyses of Certain Enteric Bacteria and Bacterial Extracts II. Discrimination of Sonic Extracts by Interfacial Densitometry of Precipitin Systems

William G. Glenn; James R. Ralston; Warren J. Russell

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Quantitative Analyses of Certain Enteric Bacteria and Bacterial Extracts II. Discrimination of Sonic Extracts by Interfacial Densitometry of Precipitin Systems

机译：某些肠道细菌和细菌提取物的定量分析II。沉淀素系统的界面密度法鉴别声波萃取物

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Bacterial extracts prepared by ultrasonic disruption were reacted with both narrow- and broad-spectrum reference (homologous) and cross-reacting (heterologous) precipitins produced in rabbits. Quantitation of the reaction was obtained by densitometry of the antigen-antibody interface. Comparisons were made of sonic extracts from various starting populations all equated to the same nitrogen concentrations, and of various nitrogen levels derived from five bacterial population levels prepared separately. Sources of error are probed to show under what circumstances cross-reactions would be of greater magnitude than reference ones. The feasibility was shown of using quantitative densitometry of the interface combined with broadly reacting precipitins to identify bacteria on an intergeneric and interspecies scale. Problems associated with the use of absorbed or monospecific precipitins are explained.

机译：通过超声破坏制备的细菌提取物与兔体内产生的窄谱和广谱参比物（同源）和交叉反应（异源）沉淀蛋白反应。通过光密度测定抗原-抗体界面获得反应的定量。比较了来自各种起始种群的声音提取物，这些提取物都等于相同的氮浓度，并且来自分别制备的五个细菌种群水平的多种氮含量。探究错误源，以表明在什么情况下交叉反应会比参考反应更严重。使用界面的定量光密度法与沉淀蛋白的广泛反应相结合来鉴定属间和种间规模细菌的可行性被证明。解释了与吸收或单特异性沉淀蛋白的使用有关的问题。

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《Applied Microbiology》 |1967年第6期|共7页
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William G. Glenn; James R. Ralston; Warren J. Russell;
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中图分类微生物学;
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1. Quantitative Analyses of Certain Enteric Bacteria and Bacterial Extracts I. Standardization and Sonic Disruption of Eight Enteric Bacterial Species, Each at Five Population Levels [J] . William G. Glenn, James R. Ralston, Warren J. Russell Applied Microbiology . 1967,第6期

机译：某些肠道细菌和细菌提取物的定量分析I.八种肠道细菌的标准化和声波破坏，每种物种在五个种群水平上
2. In Vitro Qualitative and Quantitative Phytochemical Analysis and Antibacterial Activities of Ethanolic Extracts of Anthocleista vogelii on Some Bacteria Responsible for Wound and Enteric Infection [J] . Eze. VC, Omeh. YN, Onwuakor. CE, International Journal of Pharmaceutical Sciences Review and Research . 2014,第1期

机译：伏格氏按蚊乙醇提取物的体外定性和定量植物化学分析和抗菌活性对一些负责伤口和肠道感染的细菌
3. “The application of a 3D-QSAR Approach for 7-(4H-1,2,4-Triazol-3-yl)benzo[c][2,6]naphthyridine Derivatives as PIM – 1 Inhibitors” by Shravan Kumar Gunda, Salwa Shaik, Sharada Durgam, Mahmood Shaik, India. 37 “In Vitro Qualitative and Quantitative Phytochemical Analysis and Antibacterial Activities of Ethanolic Extracts of Anthocleista vogelii on Some Bacteria Responsible for Wound and Enteric Infection” [J] . Shrabani Pradhan, Suchismita Roy, Shreya Mandal, International Journal of Pharmaceutical Sciences Review and Research . 2013,第1期

机译：“ 3D-QSAR方法在7-（4H-1,2,4-三唑-3-基）苯并[c] [2,6]萘啶衍生物作为PIM – 1抑制剂中的应用”，Shravan Kumar Gunda，Salwa Shaik，Sharada Durgam，Mahmood Shaik，印度。 37“伏格氏按蚊乙醇提取物对某些负责伤口和肠道感染的细菌的体外定性和定量植物化学分析和抗菌活性”
4. Extracting sequential episodes representing replacements of bacteria from bacterial culture data [C] . Katoh Takashi, Hirata Kouichi, Arimura Hiroki, Complex Medical Engineering, 2009. CME . 2009

机译：从细菌培养数据中提取代表细菌替换的连续事件
5. Quantitative Analyses of Certain Enteric Bacteria and Bacterial Extracts [O] . William G. Glenn, James R. Ralston, Warren J. Russell 1967

机译：某些肠细菌和细菌提取物的定量分析
6. Quantitative Precipitin Studies on Catalase Protein in Hemolysate and Acetone Extract from Acatalasemia and Hypocatalasemia [O] . Masana OGATA, Shigeo TAKAHARA 1962

机译：溶血病溶血酸盐和丙酮提取物中的定量沉淀素研究及血清血症和低钙血症

Quantitative Analyses of Certain Enteric Bacteria and Bacterial Extracts II. Discrimination of Sonic Extracts by Interfacial Densitometry of Precipitin Systems

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