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首页> 外文期刊>Applied Microbiology >Loss of Rubella Antibody from Immune Globulin Treated with Kaolin
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Loss of Rubella Antibody from Immune Globulin Treated with Kaolin

机译：高岭土治疗免疫球蛋白引起风疹抗体的流失

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摘要

Sera and immune globulin (IG) preparations are customarily treated with kaolin before titration of their rubella hemagglutination-inhibiting (HI) antibody in order to rid them of nonspecific inhibitors of hemagglutination. The treatment was shown in this investigation to have no adverse effect on the antibody level of the sera but was found to remove considerable amounts of gamma-globulin from IG preparations. Evidence of this removal was obtained by serological tests, by spectrophotometric determination of protein concentration and by disc electrophoresis. In contrast to kaolin, heparin-manganese chloride (MnCl2) treatment of IG preparations had essentially no effect on the level of antibody globulin by all the criteria used. Heparin-MnCl2-treated IG lots were in these respects similar, if not identical, to their untreated counterparts. Since nonspecific inhibitors associated with the β-lipoprotein fraction of serum are removed by the method employed to fractionate the IG samples, it seems unnecessary to treat the latter in any way for the HI test. No difficulty was encountered in this investigation with several untreated IG lots.
机译：血清和免疫球蛋白（IG）制剂通常在滴定风疹血凝抑制（HI）抗体之前用高岭土处理，以摆脱血凝素的非特异性抑制剂。该研究表明该治疗对血清抗体水平无不利影响，但发现可从IG制剂中去除大量的γ-球蛋白。通过血清学测试，通过分光光度法测定蛋白质浓度和通过圆盘电泳获得了去除的证据。与高岭土相反，按照所有使用的标准，IG制剂的肝素-氯化锰（MnCl2）处理对抗体球蛋白的水平基本上没有影响。在这些方面，肝素-MnCl2处理的IG批次与未处理的同类批次相似，即使不相同。由于与血清中β-脂蛋白部分相关的非特异性抑制剂是通过分级IG样品的方法去除的，因此似乎没有必要以任何方式对HI样品进行处理。在这项调查中，未处理的多个IG批次没有遇到困难。

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