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首页> 外文期刊>Applied Microbiology >“Spore Plate Method” for Transformation of Steroids by Fungal Spores Entrapped in Silica Gel G
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“Spore Plate Method” for Transformation of Steroids by Fungal Spores Entrapped in Silica Gel G

机译：“孢子板法”通过硅胶G中截留的真菌孢子转化类固醇

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摘要

A new technique for investigating steroid biotransformations involving the use of glucose-treated Silica Gel G thin-layer chromatography plates spotted with fungal spores and steroid substrates is described. The conversion is followed by the detection and identification of steroid metabolites and is carried out on single plates by using the spores of different fungi. During the entire process, the spores remain on the original spots and microscopical examination revealed no germination. The method was successfully applied to as little as 30 μg of substrates, and a single plate could be used to detect the steroid metabolizing activity of spores of as many as 15 different cultures.
机译：描述了一种研究类固醇生物转化的新技术，该技术涉及使用葡萄糖处理的硅胶G薄层层析板，该板上点有真菌孢子和类固醇底物。转换之后是类固醇代谢物的检测和鉴定，并通过使用不同真菌的孢子在单个平板上进行。在整个过程中，孢子保留在原始斑点上，显微镜检查未发现发芽。该方法已成功地应用于低至30μg的底物，并且一个板可用于检测多达15种不同培养物的孢子的类固醇代谢活性。

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