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Inhibition of the Plaquing Efficiency of T4r+ Bacteriophage by Subtilisin

机译：枯草杆菌蛋白酶抑制T4r +噬菌体的噬菌斑效率

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The mechanism by which the replicative cycle of T4r+ phage is inhibited by certain nonhost bacterial systems was investigated. Some Bacillaceae, especially Bacillus subtilis, decreased the plaquing efficiency of this virus more than 95% within 24 hr of exposure. Sarcina lutea and Micrococcus sp. both failed to cause any significant change in the infectivity of T4r+ phage. Preliminary investigations into the nature of the inhibitory substance(s) suggested that an extracellularly elicited protein was at least partially responsible for this effect. Further analysis has implicated subtilisin, an exoprotease from B. subtilis, as the cause of some, if not all, of the observed decrease in plaquing efficiency. Gel-filtration chromatography of control and treated 14C-labeled T4r+ phage showed a wide dispersal of phage-specific material of these particles after 24 hr of exposure to pure subtilisin or to expended medium exoprotease from B. subtilis. It was concluded that B. subtilis exoprotease is capable of chemically altering the structure of the phage capsid, thus causing a decrease in its plaquing efficiency.
机译：研究了某些非宿主细菌系统抑制T4r +噬菌体复制周期的机制。在暴露的24小时内，某些芽孢杆菌科，尤其是枯草芽孢杆菌，使该病毒的成斑效率降低了95％以上。 Sarcina lutea和Micrococcus sp。两者都未能引起T4r +噬菌体感染性的任何显着变化。对抑制物质性质的初步研究表明，细胞外诱导的蛋白质至少部分负责这种作用。进一步的分析表明枯草杆菌蛋白酶是枯草芽孢杆菌的一种外切蛋白酶，它是导致某些（如果不是全部）观察到的成斑效率下降的原因。对照和处理过的14C标记的T4r +噬菌体的凝胶过滤色谱显示，暴露于纯枯草杆菌蛋白酶或消耗枯草芽孢杆菌的培养基外蛋白酶24小时后，这些颗粒的噬菌体特异性物质广泛分散。结论是，枯草芽孢杆菌外切蛋白酶能够化学改变噬菌体衣壳的结构，从而导致其噬菌效率降低。

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