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首页> 外文期刊>Applied Microbiology >Rapid Identification of Viruses by Indirect Immunofluorescence: Isolation and Identification of Adenovirus Types 4 and 7 and Coxsackievirus Type A21 in Microcultures
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Rapid Identification of Viruses by Indirect Immunofluorescence: Isolation and Identification of Adenovirus Types 4 and 7 and Coxsackievirus Type A21 in Microcultures

机译：通过间接免疫荧光快速鉴定病毒：在微培养中分离和鉴定4型和7型腺病毒和A21型柯萨奇病毒

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摘要

The indirect fluorescent-antibody test (IFAT), employing treated and standardized antiserum in a single pool, was earlier reported to have been used successfully for the preliminary identification of nine respiratory viruses in second to ninth passage multiplying in cells propagated on microscope slides. This report describes parameters affecting the isolation in first passage and the identification by IFAT of adenovirus types 4 and 7 and coxsackievirus type A21 present in stored clinical specimens inoculated into microcultures of WI-38 cells. Isolation frequency was comparable to that obtained in tube cultures, and identification by IFAT of viruses in microcultures could be accomplished in 3 to 4 hr after recognition of a cytopathogenic effect.
机译：据报道，间接荧光抗体测试（IFAT）在单个池中采用经过处理的标准化抗血清，已成功用于第二至第九代繁殖的9种呼吸道病毒的初步鉴定，这些病毒在显微镜载玻片上繁殖的细胞中繁殖。该报告描述了影响接种于WI-38细胞微培养物的临床样本中存在的4型和7型腺病毒和A21型柯萨奇病毒的首次传代分离以及IFAT鉴定的参数。分离频率与在管培养中获得的分离频率相当，并且在识别出细胞致病作用后的3至4小时内，可以通过IFAT对微培养中的病毒进行鉴定。

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