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Technique for Differentiating Particles That Are Cell-Associated or Ingested by Macrophages

机译：巨噬细胞区分与细胞相关或摄入的颗粒的技术

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摘要

Phagocytosis is a two-step process involving attachment and ingestion of particulate material. It is often difficult to determine under a light microscope whether the particles are actually ingested or are merely attached to the cell. A more accurate, easy to perform technique with the use of xylene has been developed for determining the difference between the attachment and ingestion of polystyrene latex spheres. The xylene treatment dissolves the extracellular spheres, leaving only the intracellular spheres to be counted by the experimenter to obtain a more accurate phagocytic index. This technique also allows an investigator to get an ingestion index, an attachment index, or both.
机译：吞噬作用是一个两步过程，涉及颗粒物质的附着和摄入。在光学显微镜下通常很难确定颗粒是被实际摄入还是仅附着在细胞上。已经开发出一种使用二甲苯的更准确，易于执行的技术，用于确定聚苯乙烯乳胶球的附着与摄取之间的差异。二甲苯处理可溶解细胞外球体，仅留下细胞内球体供实验者计数以获得更准确的吞噬指数。此技术还允许研究人员获取摄入指数，附着指数或两者。

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