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Medium Without Rumen Fluid for Nonselective Enumeration and Isolation of Rumen Bacteria

机译：没有瘤胃液的培养基，用于瘤胃细菌的非选择性列举和分离

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Colony counts which approximated those in a habitat-simulating, rumen fluid-agar medium (RFM) were obtained in medium 10, a medium identical to the RFM except for the replacement of rumen fluid with 1.5 × 10-6m hemin, 0.2% Trypticase, 0.05% yeast extract, and a 6.6 × 10-2m volatile fatty acid mixture qualitatively and quantitatively similar to that in rumen fluid. Single deletion of Trypticase, yeast extract, or the volatile fatty acid mixture from medium 10 significantly reduced colony counts. Colony counts were also reduced when medium 10 was modified to contain higher concentrations of Trypticase or volatile fatty acids. Significant differences were found between colony counts obtained from diluted rumen contents of animals fed a cracked corn-urea diet, and the colony counts obtained from animals fed either a cracked corn-soyean oil meal or an alfalfa hay-grain diet. Qualitative differences were found between the predominant bacterial strains isolated from rumen contents of animals fed cracked corn diets and strains isolated from animals fed alfalfa hay-grain. Regardless of differences in the predominant flora associated with diet, medium 10 and the RFM supported growth of similar bacterial populations. The results show that medium 10 is suitable for enumeration and isolation of many predominant rumen bacteria.
机译：在培养基10中获得近似于模拟栖息地的瘤胃琼脂培养基（RFM）的菌落计数，该培养基与RFM相同，只是用1.5×10-6m血红素，0.2％的胰蛋白酶解脱瘤胃液，0.05％的酵母提取物和6.6×10-2m的挥发性脂肪酸混合物在质和量上与瘤胃液相似。从培养基10中单次删除胰蛋白酶，酵母提取物或挥发性脂肪酸混合物可显着减少菌落数。当将培养基10修改为包含更高浓度的胰蛋白酶或挥发性脂肪酸时，菌落数也减少了。发现从饲喂破裂的玉米-尿素饮食的动物的稀释瘤胃含量获得的菌落计数与从饲喂破裂的玉米-大豆油粕或苜蓿干草-谷物饮食的动物获得的菌落计数之间存在显着差异。发现从饲喂含裂纹玉米饮食的动物的瘤胃内容物中分离出的主要细菌菌株与从饲喂苜蓿干草的动物中分离出的主要细菌菌株之间存在质的差异。不论与饮食有关的主要菌群有何差异，培养基10和RFM都能支持相似细菌种群的生长。结果表明，培养基10适合于枚举和分离许多主要的瘤胃细菌。

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