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首页> 外文期刊>Applied Microbiology >Use of Immunofluorescence and Animal Tests to Detect Growth and Toxin Production by Clostridium botulinum Type E in Food
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Use of Immunofluorescence and Animal Tests to Detect Growth and Toxin Production by Clostridium botulinum Type E in Food

机译：利用免疫荧光和动物试验检测食品中E型肉毒梭菌的生长和毒素产生

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The appearance of Clostridium botulinum type E organisms and of toxin in experimentally inoculated packages of turkey roll was followed to study the time relationship between the presence of vegetative cells and the demonstration of toxin. The presence of vegetative cells was determined by immunofluorescence, and animal tests were used to assay toxin production. Growth initiated from detoxified spores of C. botulinum type E resulted in toxin formation within 24 hr. Presence of fluorescing vegetative cells and of toxin coincided from 1 to 14 days of incubation. Beginning with the next testing date, day 21, differences were observed. Toxin could be detected for a longer time than vegetative cells. Neither toxin nor organisms could be found after 56 days of incubation. The mouse lethal dose tests (MLD per gram of turkey roll) showed fluctuations in the amount of toxin present throughout the period of testing. Maximal amounts of toxin were present during the period when fluorescing organisms were also more numerous. The applications of immunofluorescence in the study and in the diagnosis of botulism is discussed.
机译：跟踪实验接种的火鸡卷包装中出现的E型肉毒梭菌生物和毒素的出现，以研究营养细胞的存在与毒素表现之间的时间关系。通过免疫荧光确定营养细胞的存在，并使用动物试验来测定毒素产生。从E型肉毒梭菌的解毒孢子开始生长，导致毒素在24小时内形成。孵育1到14天，荧光营养细胞和毒素的存在时间一致。从下一个测试日期（第21天）开始，观察到差异。与营养细胞相比，毒素的检测时间更长。孵育56天后，找不到毒素和生物。小鼠致死剂量测试（每克火鸡胶卷中的MLD值）显示了在整个测试过程中存在的毒素量的波动。在发荧光的生物也更多的期间，存在最大量的毒素。讨论了免疫荧光在肉毒中毒的研究和诊断中的应用。

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