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首页> 外文期刊>Applied Microbiology >Use of a Fluorescent Brightener to Demonstrate Cellulose in the Cellular Slime Molds
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Use of a Fluorescent Brightener to Demonstrate Cellulose in the Cellular Slime Molds

机译：荧光增白剂在细胞粘液霉菌中的纤维素用途

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The presence and location of cellulose in different stages of the life cycles of the cellular slime molds can be demonstrated by use of the disodium salt of 4,4′-bis(4-anilino-6-bis (2-hydroxyethyl)-amino-s-triazin-2-ylamino)-2,2′ -stilbene disulfonic acid, a fluorescent brightener. It may be used successfully as a direct stain at a concentration of 0.1% in half-normal saline at pH 6; and it may be incorporated into growth media as a vital stain at a concentration of 0.0025% with no inhibitory effect at any developmental stage. Vegetative myxamoebae contain no cellulose and show no fluorescence in the presence of this brightener when viewed with ultraviolet light. In later stages of the life cycle, the time and sites of cellulose formation can be demonstrated with the brightener because of its fluorescence. e.g., in the slime covering of the pseudoplasmodia, in the sorophore sheath, in the walls of stalk cells and spores, in the walls of microcysts, and in the walls and sheath material of macrocysts. The brightener appears to be a very sensitive indicator for cellulose, and it has certain advantages over other cellulose stains, since the staining reaction (fluorescence) is very intense, long-lasting, and not obscured by unstained cellulose-free myxamoebae if such are present.
机译：可以通过使用4,4'-双（4-苯胺基-6-双（2-羟乙基）-氨基- s-triazin-2-ylamino）-2,2'-sti二磺酸，荧光增白剂。在pH为6的半生理盐水中，其浓度为0.1％时，可以成功用作直接染色剂。它可以以0.0025％的浓度作为生命染色剂掺入生长培养基中，在任何发育阶段均无抑制作用。营养性粘藻科不含纤维素，在紫外线下观察该增白剂时无荧光。在生命周期的后期，由于其荧光性，可以用增白剂证明纤维素形成的时间和部位。例如，在假疟原虫的粘液中，在荧光团鞘中，在茎细胞和孢子的壁中，在微囊的壁中以及在大囊的壁和鞘中。增白剂似乎是纤维素的非常敏感的指示剂，并且与其他纤维素染色剂相比，具有某些优势，因为染色反应（荧光）非常强烈，持久且不会被未染色的不含纤维素的甲霜菌所掩盖。 。

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