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Production of Bacterial Cells from Methane

机译：由甲烷生产细菌细胞

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A mixed methane-oxidizing bacterial culture capable of stable and predictable growth in continuous culture was isolated. The culture consisted of two types of gram-negative nonsporulating rods resembling pseudomonads. The culture grew well at 45 C on an inorganic medium without asepsis. Specific metal requirements for Ca2+, Cu2+, MoO42?, Zn2+, Mn2+, Mg2+, and Fe3+ (or Fe2+) were shown. The cells grown in continuous culture contained 11.7 to 12.1% total nitrogen. From an animal nutrition standpoint, the distribution of amino acids was satisfactory. The continuous fermentation was operated over a range of steady-state dilution rates from 0.085 to 0.301 hr?1. The maximum specific growth rate for the culture, μmax, was 0.303 hr?1 (doubling time 2.29 hr). The average yield for all fermentations analyzed was 0.616 g (dry weight of cells per g of methane used and 0.215 g (dry weight) of cells per g of oxygen used. The yields on both methane and oxygen were higher for the oxygen-limited than for the methane-limited fermentations. The maximum productivity attained in the fermentor was 2.39 g (dry weight) of cells per hr per liter at a dilution rate of 0.187 hr?1 and a cell concentration of 12.8 g (dry weight) of cells per liter. The limit on maximum cell productivity was determined only by the mass transfer rate of oxygen in the fermentor. The simultaneous volumetric mass-transfer coefficients (kLa in hr?1) for oxygen and methane were determined. The results appear to indicate an oxygen to methane mass-transfer coefficient ratio of approximately 1.4.
机译：分离出能够在连续培养中稳定且可预测地生长的混合甲烷氧化细菌培养物。该培养物由类似于假单胞菌的两种革兰氏阴性无孢子杆组成。培养物在无无菌的无机培养基上于45°C良好生长。示出了Ca 2+，Cu 2+，MoO 4 2，Zn 2+，Mn 2+，Mg 2+和Fe 3+（或Fe 2+）的特定金属需求。在连续培养中生长的细胞含有11.7至12.1％的总氮。从动物营养的角度来看，氨基酸的分布令人满意。连续发酵在从0.085到0.301 hr？1的稳态稀释率范围内进行。培养物的最大比生长率μmax为0.303hr？1（倍增时间2.29hr）。分析的所有发酵的平均产量为0.616 g（每克所用甲烷的细胞干重和0.215 g（每克所用氧气的细胞干重）。限氧的甲烷和氧气的产率均高于对于甲烷限制的发酵，发酵罐中获得的最大生产率为2.39 g（干重）细胞/小时/升，稀释率为0.187 hr？1，细胞浓度为12.8 g（干重）/细胞。仅通过发酵罐中氧气的传质速率确定最大细胞生产率的极限，同时测定氧气和甲烷的体积传质系数（kLa in hr？1），结果似乎表明有氧气与甲烷的传质系数之比约为1.4。

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