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Vibrio parahaemolyticus Gastroenteritis in Maryland: Laboratory Aspects

机译:马里兰州副溶血性弧菌肠胃炎:实验室方面

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Vibrio parahaemolyticus was the etiological agent in three food-related epidemics of gastroenteritis in Maryland, during August 1971. These outbreaks involved crab food products. Fifteen isolates of V. parahaemolyticus were made which included 11 from patients and 4 from foods. Serotype 04:K11 was the cause of the outbreaks. It was recovered from patients in each outbreak and gave a positive Kanagawa reaction, an indication of enteropathogenicity. Other patient isolates included types 03:K30, 03:K33, and an untypable isolate, all of which were Kanagawa negative. Food isolates included serotypes 03:K30, 02:K28, and two untypable isolates, all of which were Kanagawa negative. The outbreaks reported in this paper constitute the first confirmed foodborne epidemics due to V. parahaemolyticus in the United States. Methods for the isolation and identification of V. parahaemolyticus are presented, including a procedure for the simple conversion of conventional laboratory media into suitable culture media for this halophilic organism.
机译:1971年8月,副溶血性弧菌是马里兰州3种与食物有关的胃肠炎流行病的病原体。这些暴发涉及蟹类食品。分离出十五株副溶血性弧菌,其中包括来自患者的11株和来自食物的4株。血清型04:K11是爆发的原因。每次暴发都从患者身上回收,神奈川反应呈阳性,表明有肠道致病性。其他患者分离株包括类型03:K30、03:K33和不可分离的分离株,所有分离株均为神奈川阴性。食物分离株包括血清型03:K30、02:K28和两个无法分型的分离株,所有分离株均为神奈川阴性。本文报道的暴发是美国首次因副溶血性弧菌确诊的食源性流行病。提出了分离和鉴定溶血性弧菌的方法,包括将常规实验室培养基简单转化为该嗜盐生物的合适培养基的方法。

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