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首页> 外文期刊>Applied Microbiology >Direct Fluorescent-Antibody Method for the Diagnosis of Pneumocystis carinii Pneumonitis from Sputa or Tracheal Aspirates from Humans
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Direct Fluorescent-Antibody Method for the Diagnosis of Pneumocystis carinii Pneumonitis from Sputa or Tracheal Aspirates from Humans

机译：直接荧光抗体法诊断人痰中的卡氏肺孢子虫肺炎或人的气管抽吸物

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A direct fluorescent antibody (DFA) method was applied to sputum or tracheal aspirate from 68 patients with clinical or radiological evidence suggesting Pneumocystis carinii pneumonitis, and to 50 control patients. P. carinii was detected by DFA in specimens from 33 of the 69 clinical cases and 3 of the 50 controls. Specimens of lung from 11 of 33 DFA-positive cases were examined histologically, and 9 were positive. Four of 35 DFA-negative cases were examined histologically, and all were negative. Sputa or tracheal aspirates from 6 patients who were positive by both DFA and histological examination were examined also by methenamine silver staining; none could be diagnosed conclusively by this method. The results indicate that the DFA method is a sensitive and dependable procedure for the laboratory diagnosis of P. carinii pneumonitis in man.
机译：将直接荧光抗体（DFA）方法应用于68例临床或放射学证据表明卡氏肺孢子虫性肺炎的患者的痰或气管抽吸物，以及50例对照患者。用DFA在69例临床病例中的33例和50例对照中的3例标本中检出了卡氏疟原虫。组织学检查了33例DFA阳性病例中11例的肺标本，其中9例阳性。对35例DFA阴性病例中的4例进行了组织学检查，均为阴性。通过DFA和组织学检查均阳性的6例患者的痰或气管抽吸物也通过二甲胺银染色进行了检查;用这种方法无法最终诊断。结果表明，DFA方法是一种用于实验室诊断人卡氏肺炎的敏感而可靠的方法。

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