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Hydrogen Sulfide Production by Pseudomonas putrefaciens in Shrimp Experimentally Packed in Nitrogen

机译:实验性用氮气包装的虾中的恶臭假单胞菌生产硫化氢

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Shrimp refrigerated in a nitrogen atmosphere develop off-odors not typical of normal spoilage. Investigations of this phenomenon showed that hydrogen sulfide developed in the headspace gas, and a large percentage of the microbial population present on the shrimp stored in nitrogen was capable of hydrogen sulfide production, in contrast to the flora on shrimp stored in air. The predominant hydrogen sulfide-producing organism, Pseudomonas putrefaciens, was present in low numbers on fresh shrimp but usually reached high numbers by day 8 of nitrogen storage. Further studies revealed that cysteine and cystine were the probable substrates in shrimp utilized by this organism for hydrogen sulfide production. When shrimp sterilized by irradiation were inoculated with P. putrefaciens and incubated in an atmosphere of nitrogen, hydrogen sulfide and the characteristic off-odors developed.
机译:在氮气氛围中冷藏的虾会散发出通常不常见的异味。对这种现象的研究表明,与储存在空气中的对虾的菌群相反,在顶空气体中产生了硫化氢,存在于氮中的对虾中存在的大部分微生物种群能够产生硫化氢。盛产硫化氢的主要生物腐烂假单胞菌(Pseudomonas putrefaciens)在鲜虾上的数量很少,但到氮存储第8天通常达到很高的数量。进一步的研究表明,半胱氨酸和胱氨酸是该生物体用于生产硫化氢的虾中可能的底物。当用辐照灭菌的虾接种腐烂假单胞菌并在氮气,硫化氢气氛中孵育时,会产生典型的异味。

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