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Effect of Osmotic Stabilizers on 14CO2 Production by Bacteria and Blood

机译：渗透稳定剂对细菌和血液产生14CO2的影响

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Evolution of 14CO2 by whole blood as well as by Diplococcus pneumoniae, Haemophilus sp., Pseudomonas aeruginosa, Pseudomonas diminuta, and Streptococcus pyogenes was examined by using the BACTEC system. The control medium was JLI no. 6A culture vial containing 30 ml of enriched tryptic soy broth and 1.5 μCi of 14C-substrate. Hypertonic media consisted of control medium with either 1 or 3% NaCl, 10% sucrose, and 5%, 10%, or 15% dextran. The most deleterious treatment to bacteria was 3% NaCl since it not only retarded 14CO2 production, but also prevented growth of D. pneumoniae, Haemophilus, and P. diminuta. The 10% sucrose treatment diminished 14CO2 output, although it did not retard growth of test organisms. This effect was probably due to 14C-substrate dilution rather than to osmotic effects. Dextran had slight effect on 14CO2 production and slightly acidified the medium. Of the treatments tested, only 10% sucrose reduced normal output of 14CO2 by whole blood. This also is probably due to 14C-substrate dilution. It appears that 10% sucrose is potentially the most useful osmotic agent for radiometric techniques since, although bacterial 14CO2 production is lowered, blood 14CO2 is lowered also.
机译：使用BACTEC系统检查全血以及肺炎双球菌，嗜血杆菌属，铜绿假单胞菌，假单胞菌和化脓链球菌的14CO2释放。对照培养基是JLI no。 6A培养瓶，其中包含30 ml富含胰蛋白酶的大豆肉汤和1.5μCi的14C底物。高渗培养基由含有1％或3％NaCl，10％蔗糖和5％，10％或15％右旋糖酐的对照培养基组成。对细菌最有害的处理方法是3％NaCl，因为它不仅会延迟14CO2的产生，而且会阻止肺炎衣原体，嗜血杆菌和小球菌的生长。 10％的蔗糖处理减少了14CO2的输出，尽管它不会延迟测试生物的生长。这种作用可能是由于14C底物稀释而不是渗透作用所致。右旋糖酐对14CO2的产生有轻微的影响，并使培养基略微酸化。在所测试的治疗方法中，只有10％的蔗糖降低了全血的正常14CO2输出量。这也可能是由于14C底物稀释。似乎10％的蔗糖可能是放射技术最有用的渗透剂，因为尽管细菌14CO2的产生量降低了，但血液14CO2的含量也降低了。

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