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Some Factors Affecting the Activity of Diethylpyrocarbonate as a Sterilant

机译：影响焦碳酸二乙酯杀菌剂活性的一些因素

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摘要

Quantitative data indicated logarithmic death in 5° Brix Concord grape juice when concentrations of cells under 107/ml were exposed to diethylpyrocarbonate (DEPC). Species differed considerably in their resistance; e.g., 50 ppm reduced the viable count of Saccharomyces cerevisiae over nine log10 cycles, whereas 200 ppm reduced the count of Byssochlamys fulva ascospores by only about 1 log. DEPC lethality was enhanced by higher temperatures; destruction at 40 C was 10- to 100-fold greater than at 20 C. Studies on death rates showed that most yeasts and fungal spores were killed during the first hour of exposure, whereas 24 h or longer was needed for maximal destruction of several lactic acid bacteria. Repair of DEPC-induced damage was believed responsible for the slower death rates of the lactics.
机译：定量数据表明，当浓度低于107 / ml的细胞暴露于焦碳酸二乙酯（DEPC）时，在5°Brix Concord葡萄汁中对数死亡。物种的抗性差异很大。例如，在9个log10周期内，50 ppm减少了酿酒酵母的存活数，而200 ppm减少了Byssochlamys fulva子囊孢子的数量仅约1 log。较高的温度提高了DEPC的杀伤力;在40 C时的破坏比在20 C时大10到100倍。关于死亡率的研究表明，大多数酵母和真菌孢子在接触的第一小时内被杀死，而要最大限度地破坏几种乳酸则需要24小时或更长时间酸性细菌。据信，修复DEPC引起的损害是造成乳酸降低死亡率的原因。

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