Comparison of Rapid Methods for Analysis of Bacterial Fatty Acids

C. Wayne Moss; M. A. Lambert; W. H. Merwin

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Comparison of Rapid Methods for Analysis of Bacterial Fatty Acids

机译：细菌脂肪酸快速分析方法的比较

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When rapid gas-liquid chromatography methods for determination of bacterial fatty acids were compared, results showed that saponification was required for total fatty acid analysis. Transesterification with boron-trihalide reagents (BF3-CH3OH, BCl3-CH3OH) caused extensive degradation of cyclopropane acids and was less effective than saponification in releasing cellular hydroxy fatty acids. Digestion of cells with tetramethylammonium hydroxide was unsatisfactory because of extraneous gas-liquid chromatography peaks and because of lower recovery of branched-chain and hydroxy fatty acids. A simple, rapid saponification procedure which can be used for total cellular fatty acid analysis of freshly grown cells is described.

机译：当比较快速气液色谱法测定细菌脂肪酸时，结果表明皂化是总脂肪酸分析所必需的。用三卤化硼试剂（BF3-CH3OH，BCl3-CH3OH）进行酯交换反应会引起环丙烷酸的广泛降解，并且在释放细胞羟基脂肪酸方面不如皂化反应有效。用氢氧化四甲铵消化细胞的效果不理想，这是因为外部的气相色谱峰，以及支链和羟基脂肪酸的回收率较低。描述了一种可用于新鲜生长细胞的总细胞脂肪酸分析的简单，快速的皂化过程。

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《Applied Microbiology》 |1974年第1期|共6页
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C. Wayne Moss; M. A. Lambert; W. H. Merwin;
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1. Strain-level bacterial identification by CeO₂-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods [J] . C. R. Cox, K. R. Jensen, N. R. Saichek, Scientific reports. . 2015,第1期

机译：CeO _{2 催化的MALDI-TOF MS脂肪酸分析对菌株级细菌的鉴定以及与基于商业蛋白质的方法的比较}
2. Strain-level bacterial identification by CeO₂-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods [J] . C. R. Cox, K. R. Jensen, N. R. Saichek, Scientific reports. . 2015,第1期

机译：CeO _{2 催化的MALDI-TOF MS脂肪酸分析对菌株级细菌的鉴定以及与基于商业蛋白质的方法的比较}
3. Comparison of Two Derivatization Methods for the Analysis of Fatty Acids andTransFatty Acids in Bakery Products Using Gas Chromatography [J] . JumatSalimon, Talal A.Omar, NadiaSalih ScientificWorldJournal . 2014,第3期

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4. A Rapid Method for Estimating Triglyceride Synthesis in vivo Using Stable Labeled Fatty Acids and analysis by UPLC-MS/MS [C] . Timothy He, David McLaren, Sheng-Ping Wang, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

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5. Fatty acid profiling of soil microbial communities: A comparison of extraction methods and temporal dynamics in plant residue amended soils . [D] . Fernandes, Marcelo Ferreira. 2006

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6. Comparison of Rapid Methods for Analysis of Bacterial Fatty Acids [O] . C. Wayne Moss, M. A. Lambert, W. H. Merwin 1974

机译：细菌脂肪酸快速分析方法的比较
7. Strain-level bacterial identification by CeO2-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods [O] . C. R. Cox, K. R. Jensen, N. R. Saichek, 2015

机译：CeO2催化MARDI-TOF MS脂肪酸分析的应变水平细菌鉴定及商业蛋白质方法的比较

Comparison of Rapid Methods for Analysis of Bacterial Fatty Acids

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