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首页> 外文期刊>Applied Microbiology >Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium
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Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium

机译：木腐真菌胎盘和Phanerochaete chrysosporium的比较转录组和分泌组分析

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摘要

Cellulose degradation by brown rot fungi, such as Postia placenta , is poorly understood relative to the phylogenetically related white rot basidiomycete, Phanerochaete chrysosporium . To elucidate the number, structure, and regulation of genes involved in lignocellulosic cell wall attack, secretome and transcriptome analyses were performed on both wood decay fungi cultured for 5 days in media containing ball-milled aspen or glucose as the sole carbon source. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), a total of 67 and 79 proteins were identified in the extracellular fluids of P. placenta and P. chrysosporium cultures, respectively. Viewed together with transcript profiles, P. chrysosporium employs an array of extracellular glycosyl hydrolases to simultaneously attack cellulose and hemicelluloses. In contrast, under these same conditions, P. placenta secretes an array of hemicellulases but few potential cellulases. The two species display distinct expression patterns for oxidoreductase-encoding genes. In P. placenta , these patterns are consistent with an extracellular Fenton system and include the upregulation of genes involved in iron acquisition, in the synthesis of low-molecular-weight quinones, and possibly in redox cycling reactions.
机译：相对于与系统发育相关的白色腐烂担子菌，Phanerochaete chrysosporium而言，人们对棕色腐烂真菌（例如，Postia胎盘）降解纤维素的了解很少。为了阐明参与木质纤维素细胞壁攻击的基因的数量，结构和调控，对在含有球磨白杨木或葡萄糖作为唯一碳源的培养基中培养5天的木材腐烂真菌进行了分泌组和转录组分析。使用液相色谱-串联质谱法（LC-MS / MS），在胎盘衣原体和金黄色葡萄球菌培养物的细胞外液中分别鉴定出总共67和79种蛋白质。与转录物谱一起观察，金孢假单胞菌利用一系列细胞外糖基水解酶同时攻击纤维素和半纤维素。相反，在这些相同条件下，胎盘疟原虫分泌一系列半纤维素酶，但潜在的纤维素酶很少。这两个物种显示出不同的氧化还原酶编码基因表达模式。在胎盘疟原虫中，这些模式与细胞外Fenton系统一致，包括上调参与铁摄取，低分子量醌的合成以及可能在氧化还原循环反应中的基因的上调。

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