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首页> 外文期刊>Applied Microbiology >Solid Phase Radioimmunoassay for Identification of Herpesvirus hominis Types 1 and 2 from Clinical Materials
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Solid Phase Radioimmunoassay for Identification of Herpesvirus hominis Types 1 and 2 from Clinical Materials

机译：固相放射免疫法从临床材料中鉴定人疱疹病毒1型和2型

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A solid phase radioimmunoassay (RIA) was developed for typing Herpesvirus hominis (HVH) strains isolated from clinical materials, and it also proved to be applicable to the direct detection and typing of HVH antigen in human and animal brain tissue. The procedure utilized virus-infected human fetal diploid cells or brain tissue smears in the bottom of 1-dram glass vials, antigen was detected through the use of intermediate HVH antisera produced in rabbits or hamsters and cross-absorbed with the HVH heterotype, and 125I-labeled anti-species (rabbit or hamster) globulins produced in goats were used for detection of immune complexes. The cross-absorbed HVH antisera could be used at high dilutions in the RIA test, and they reacted with marked type-specificity in the RIA system. Specificity of the test was also improved by determining and using optimal concentrations of intermediate sera and of 125I-labeled anti-species globulins. Results of typing HVH isolates by the RIA procedure agreed in all instances with those obtained by direct fluorescent antibody staining with cross-absorbed conjugates. The RIA procedure was effective and more sensitive than direct fluorescent antibody for demonstrating and typing HVH antigen directly in smears of infected human brain tissue.
机译：开发了一种固相放射免疫分析法（RIA），用于分型从临床材料分离的人疱疹病毒（HVH）菌株，它也被证明可用于在人和动物脑组织中直接检测和分型HVH抗原。该程序利用病毒感染的人胎儿二倍体细胞或1缸玻璃小瓶底部的脑组织涂片，通过使用在兔或仓鼠中产生并与HVH异型交叉吸收的中间HVH抗血清检测抗原用山羊生产的标记的抗物种（兔或仓鼠）球蛋白检测免疫复合物。交叉吸收的HVH抗血清可以在RIA测试中以高稀释度使用，并且它们在RIA系统中以明显的类型特异性反应。通过确定和使用中间血清和125 I标记的抗种球蛋白的最佳浓度，还可以提高测试的特异性。在所有情况下，通过RIA程序对HVH分离株进行分型的结果与通过交叉吸收的偶联物直接进行荧光抗体染色获得的结果一致。对于直接在被感染的人脑组织涂片中证实和分型HVH抗原，RIA程序比直接荧光抗体更有效且更敏感。

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