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首页> 外文期刊>Applied Microbiology >Use of Immunoperoxidase for the Rapid Identification of Human Myxoviruses and Paramyxoviruses in Tissue Culture
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Use of Immunoperoxidase for the Rapid Identification of Human Myxoviruses and Paramyxoviruses in Tissue Culture

机译：免疫过氧化物酶在组织培养中快速鉴定人类黏病毒和副黏病毒的应用

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The immunoperoxidase method, using commercially available antisera, was compared with standard virological methods for the identification and typing of 77 isolates of human myxoviruses and paramyxoviruses. Results of typing using neutralization tests and the immunoperoxidase technique were identical for 76 of the 77 isolates. With the immunoperoxidase method there was one false negative reaction, but no false positive reactions. Cross-reactivity between influenza A (soluble) and A2/HK antisera with influenza A isolates was noted, but did not interfere with the interpretation of results. It is concluded that the immunoperoxidase method is ideally suited for the rapid identification and typing of common human respiratory viruses on a routine basis. It offers a number of decided advantages over both immunofluorescence and standard virological methods.
机译：将使用市售抗血清的免疫过氧化物酶方法与标准病毒学方法进行比较，以鉴定和分型人类粘液病毒和副粘液病毒的77种分离株。使用中和测试和免疫过氧化物酶技术进行分型的结果与77个分离株中的76个相同。使用免疫过氧化物酶方法，只有一个假阴性反应，但没有假阳性反应。注意到甲型流感病毒（可溶性）和A2 / HK抗血清与甲型流感病毒分离株之间有交叉反应，但并不干扰结果的解释。结论是，免疫过氧化物酶方法非常适合常规常规快速鉴定和分型人类呼吸道病毒。与免疫荧光法和标准病毒学方法相比，它具有许多确定的优势。

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