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首页> 外文期刊>Applied Microbiology >Immunofluorescent Cell-Counting Assay for Lymphocytic Choriomeningitis Virus
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Immunofluorescent Cell-Counting Assay for Lymphocytic Choriomeningitis Virus

机译：淋巴细胞绒毛膜脑膜炎病毒的免疫荧光细胞计数测定

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摘要

A quantitative assay for lymphocytic choriomeningitis virus was developed and standardized. The assay is based on direct immunofluorescent staining of infected L-929 cell monolayers and enumeration of cells containing fluorescent viral antigens. Maximal adsorption of virus to cells occurred within 1 h. Observations on the sequential development of viral antigens within cells showed that specific cytoplasmic fluorescence appeared within 10 h. The optimal time for enumerating fluorescent cells was from 18 to 20 h after addition of virus. A linear relationship was demonstrated between the number of infected cells and the relative virus concentration. Fluorescent cells were distributed randomly in infected cover slip cell monolayers. The immunofluorescent cell-counting assay for lymphocytic choriomeningitis virus was highly precise and reproducible.
机译：淋巴细胞性脉络膜脑膜炎病毒的定量测定方法已开发并标准化。该测定基于感染的L-929细胞单层的直接免疫荧光染色和包含荧光病毒抗原的细胞计数。病毒在1小时内最大程度地吸附到细胞上。对细胞内病毒抗原顺序生长的观察表明，特定的细胞质荧光在10小时内出现。加入病毒后，枚举荧光细胞的最佳时间为18至20小时。感染细胞的数量与相对病毒浓度之间存在线性关系。荧光细胞随机分布在感染的盖玻片单层中。淋巴细胞性脉络膜脑膜炎病毒的免疫荧光细胞计数测定法非常精确且可重复。

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