Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection

Chenggang Wu; Hung Ton-That

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Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection

机译：放线菌的等位基因交换与mCherry荧光反选择

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Described here is a method for facile generation of markerless gene deletion mutants of Actinomyces oris . Homologous integration of a nonreplicative vector carrying a gene exchange cassette into the bacterial chromosome was selected for by using mCherry fluorescence and resistance to kanamycin. Completion of allelic replacement was counterselected for by using loss of fluorescence.

机译：这里描述的是一种简便的产生放线菌的无标记基因缺失突变体的方法。通过使用mCherry荧光和对卡那霉素的抗性选择携带基因交换盒的非复制载体的同源整合到细菌染色体中。通过使用荧光损失来反选择等位基因置换的完成。

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《Applied Microbiology》 |2010年第17期|共3页
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Chenggang Wu; Hung Ton-That;
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中图分类微生物学;
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1. Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection [J] . Chenggang Wu, Hung Ton-That Applied Microbiology . 2010,第17期

机译：放线菌的等位基因交换与mCherry荧光反选择
2. Evidence for recombination between a sialidase (nanH) of Actinomyces naeslundii and Actinomyces oris, previously named 'Actinomyces naeslundii genospecies 1 and 2' [J] . Do T, Henssge U, Gilbert SC, FEMS Microbiology Letters . 2008,第2期

机译：内生放线菌和先前命名为“内生放线菌1和2”的放线菌唾液酸酶（nanH）重组的证据
3. Evidence for recombination between a sialidase (nanH) of Actinomyces naeslundii and Actinomyces oris, previously named 'Actinomyces naeslundii genospecies 1 and 2' [J] . Do T, Henssge U, Gilbert SC, FEMS Microbiology Letters . 2008,第2期

机译：内生放线菌和先前命名为“内生放线菌1和2”的放线菌唾液酸酶（nanH）重组的证据
4. DEALING WITH SEQUENCE VARIABILITY DURING THE COMPARATIVE HYDROGEN EXCHANGE MASS SPECTROMETRY ANALYSIS OF MULTIPLE ALLELIC VARIANTS [C] . Thomas E. Wales, Lori Emert-Sedlak, Thomas E. Smithgall, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：在多个等位基因变体对比较氢交换质谱分析中处理序列变异性
5. Detection of superoxide anion radicals in anion exchange membrane fuel cells using in-situ fluorescence spectroscopy. [D] . Zhang, Yunzhu. 2016

机译：使用原位荧光光谱法检测阴离子交换膜燃料电池中的超氧阴离子自由基。
6. Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection [O] . Chenggang Wu, Hung Ton-That 2010

机译：放线菌的等位基因交换与mCherry荧光反选择
7. Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection ▿ [O] . Wu, Chenggang, Ton-That, Hung 2010

机译：放线菌的等位基因交换与mCherry荧光反选择
8. Pathogenicity of Exopolysaccharide-Producing Actinomyces oris Isolated from an Apical Abscess Lesion. [R] . Yamane, K., Nambu, T., Yamanaka, T., 2013

机译：从顶端脓肿病变中分离出产胞外多糖的放线菌的致病性。

Allelic Exchange in Actinomyces oris with mCherry Fluorescence Counterselection

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