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首页> 外文期刊>Applied Microbiology >Expanded Multilocus Sequence Typing and Comparative Genomic Hybridization of Campylobacter coli Isolates from Multiple Hosts
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Expanded Multilocus Sequence Typing and Comparative Genomic Hybridization of Campylobacter coli Isolates from Multiple Hosts

机译：扩展的多基因座序列分型和来自多个宿主的弯曲杆菌分离株的比较基因组杂交

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The purpose of this work was to evaluate the evolutionary history of Campylobacter coli isolates derived from multiple host sources and to use microarray comparative genomic hybridization to assess whether there are particular genes comprising the dispensable portion of the genome that are more commonly associated with certain host species. Genotyping and ClonalFrame analyses of an expanded 16-gene multilocus sequence typing (MLST) data set involving 85 isolates from 4 different hosts species tentatively supported the development of C. coli host-preferred groups and suggested that recombination has played various roles in their diversification; however, geography could not be excluded as a contributing factor underlying the history of some of the groups. Population genetic analyses of the C. coli pubMLST database by use of STRUCTURE suggested that isolates from swine form a relatively homogeneous genetic group, that chicken and human isolates show considerable genetic overlap, that isolates from ducks and wild birds have similarity with environmental water samples and that turkey isolates have a connection with human infection similar to that observed for chickens. Analysis of molecular variance (AMOVA) was performed on these same data and suggested that host species was a significant factor in explaining genetic variation and that macrogeography (North America, Europe, and the United Kingdom) was not. The microarray comparative genomic hybridization data suggested that there were combinations of genes more commonly associated with isolates derived from particular hosts and, combined with the results on evolutionary history, suggest that this is due to a combination of common ancestry in some cases and lateral gene transfer in others.
机译：这项工作的目的是评估源自多个宿主来源的弯曲杆菌分离株的进化历史，并使用微阵列比较基因组杂交来评估是否存在某些特定基因，这些特定基因包含基因组的可分配部分，而这些基因通常与某些宿主物种相关。对扩展的16基因多基因序列类型（MLST）数据集进行基因分型和ClonalFrame分析，该数据集涉及来自4种不同宿主物种的85个分离株，初步支持了大肠杆菌宿主优选组的发展，并表明重组在其多样化中发挥了多种作用;但是，不能排除地理因素是某些群体历史的基础。使用STRUCTURE对C. coli pubMLST数据库进行的群体遗传学分析表明，猪分离株形成相对同质的遗传群体，鸡和人分离株显示出相当大的遗传重叠，鸭和野鸟分离株与环境水样具有相似性。火鸡分离物与人类感染有相似之处，类似于对鸡的观察。对这些相同数据进行了分子变异分析（AMOVA），表明宿主物种是解释遗传变异的重要因素，而宏观地理环境（北美，欧洲和英国）则不是。微阵列比较基因组杂交数据表明，存在更多与特定宿主分离株相关的基因组合，并结合进化史的结果表明，这是由于某些情况下共同祖先和侧向基因转移的组合在其他人。

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