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首页> 外文期刊>Applied Microbiology >Distribution of Antimicrobial Resistance and Virulence Genes in Enterococcus spp. and Characterization of Isolates from Broiler Chickens
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Distribution of Antimicrobial Resistance and Virulence Genes in Enterococcus spp. and Characterization of Isolates from Broiler Chickens

机译：肠球菌中抗菌素耐药性和毒力基因的分布肉鸡分离株的分离与鉴定

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Enterococci are now frequent causative agents of nosocomial infections. In this study, we analyzed the frequency and distribution of antibiotic resistance and virulence genotypes of Enterococcus isolates from broiler chickens. Fecal and cecal samples from nine commercial poultry farms were collected to quantify total enterococci. Sixty-nine presumptive enterococci were isolated and identified by API 20 Strep, and their susceptibilities to antibiotics were determined. Genotypes were assessed through the use of a novel DNA microarray carrying 70 taxonomic, 17 virulence, and 174 antibiotic resistance gene probes. Total enterococcal counts were different from farm to farm and between sample sources ( P < 0.01). Fifty-one (74%) of the isolates were identified as E. faecium , whereas nine (13%), seven (10%), and two (3%) isolates were identified as E. hirae , E. faecalis , and E. gallinarum , respectively. Multiple-antibiotic resistance was evident in E. faecium and E. faecalis isolates. The most common multiple-antibiotic resistance phenotype was Bac Ery Tyl Lin Str Gen Tet Cip. Genes conferring resistance to aminoglycoside ( aac , aacA-aphD , aadB , aphA , sat4 ), macrolide ( ermA , ermB , ermAM , msrC ), tetracycline ( tetL , tetM , tetO ), streptogramin ( satG _ vatE8 ), bacitracin ( bcrR ), and lincosamide ( linB ) antibiotics were detected in corresponding phenotypes. A range of 9 to 12 different virulence genes was found in E. faecalis , including ace , agg , agrB_(Efs) ( agrB gene of E. faecalis ), cad1 , the cAM373 and cCF10 genes, cob , cpd1 , cylAB , efaA_(Efs) , and gelE . All seven E. faecalis isolates were found to carry the gelE gene and to hydrolize gelatin and bile salts. Results from this study showed the presence of enterococci of public and environmental health concerns in broiler chicken farms and demonstrated the utility of a microarray to quickly and reliably analyze resistance and virulence genotypes of Enterococcus spp.
机译：肠球菌现在是医院感染的常见病原体。在这项研究中，我们分析了肉鸡肠球菌分离株的抗生素耐药性和毒力基因型的频率和分布。收集了来自九个商业家禽场的粪便和盲肠样品，以定量总肠球菌。通过API 20 Strep分离并鉴定了69个推测性肠球菌，并确定了它们对抗生素的敏感性。通过使用携带70种生物分类，17种毒力和174种抗生素抗性基因探针的新型DNA微阵列评估基因型。农场之间和样本来源之间的总肠球菌计数不同（P <0.01）。分离株中有51个（74％）被鉴定为粪肠球菌，而分离株中有9个（13％），7个（10％）和2个（3％）分离株被鉴定为平肠埃希氏菌，粪肠球菌和大肠杆菌。 Gallinarum分别。在粪肠球菌和粪肠球菌分离物中，对多种抗生素具有抗性。最常见的多重抗生素耐药性表型是Bac Ery Tyl Lin Str Gen Tet Cip。赋予对氨基糖苷（aac，aacA-aphD，aadB，aphA，sat4），大环内酯（ermA，ermB，ermAM，msrC），四环素（tetL，tetM，tetO），链蛋白（satG_ vatE8），杆菌肽（bacitracin）抗性的基因，并在相应的表型中检测到了林可酰胺（linB）抗生素。在粪肠球菌中发现了9到12种不同的毒力基因，包括ace，agg，agrB_（Efs）（粪肠球菌的agrB基因），cad1，cAM373和cCF10基因，cob，cpd1，cylAB，efaA_（ Efs）和gelE。发现所有七个粪肠球菌分离物均携带gelE基因并水解明胶和胆汁盐。这项研究的结果表明，肉鸡场中存在对公共卫生和环境卫生的关注的肠球菌，并证明了微阵列可用于快速，可靠地分析肠球菌的抗性和毒力基因型。

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