...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporiopsis subvermispora during Growth on Aspen Wood Reveal This Organism's Strategy for Degrading Lignocellulose
【24h】

Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporiopsis subvermispora during Growth on Aspen Wood Reveal This Organism's Strategy for Degrading Lignocellulose

机译：在白杨木生长过程中选择性木质素分解真菌Ceriporiopsis subvermispora分泌组的时间变化揭示了该生物降解木质纤维素的策略。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The white-rot basidiomycetes efficiently degrade all wood cell wall polymers. Generally, these fungi simultaneously degrade cellulose and lignin, but certain organisms, such as Ceriporiopsis subvermispora, selectively remove lignin in advance of cellulose degradation. However, relatively little is known about the mechanism of selective ligninolysis. To address this issue, C. subvermispora was grown in liquid medium containing ball-milled aspen, and nano-liquid chromatography-tandem mass spectrometry was used to identify and estimate extracellular protein abundance over time. Several manganese peroxidases and an aryl alcohol oxidase, both associated with lignin degradation, were identified after 3 days of incubation. A glycoside hydrolase (GH) family 51 arabinofuranosidase was also identified after 3 days but then successively decreased in later samples. Several enzymes related to cellulose and xylan degradation, such as GH10 endoxylanase, GH5_5 endoglucanase, and GH7 cellobiohydrolase, were detected after 5 days. Peptides corresponding to potential cellulose-degrading enzymes GH12, GH45, lytic polysaccharide monooxygenase, and cellobiose dehydrogenase were most abundant after 7 days. This sequential production of enzymes provides a mechanism consistent with selective ligninolysis by C. subvermispora.
机译：白腐烂担子菌可有效降解所有木质细胞壁聚合物。通常，这些真菌同时降解纤维素和木质素，但是某些生物体，例如Ceriporiopsis subvermispora，在纤维素降解之前会选择性地去除木质素。然而，关于选择性木质素分解的机制知之甚少。为了解决这个问题，在含球磨白杨的液体培养基中培养了C. subvermispora，并使用纳米液相色谱-串联质谱法鉴定和估计了一段时间后细胞外蛋白质的丰度。孵育3天后，鉴定出了几种与木质素降解有关的锰过氧化物酶和芳基醇氧化酶。 3天后也鉴定出了糖苷水解酶（GH）家族51阿拉伯呋喃糖苷酶，但随后的样品中却逐渐减少。 5天后检测到几种与纤维素和木聚糖降解相关的酶，例如GH10内切木聚糖酶，GH5_5内切葡聚糖酶和GH7纤维二糖水解酶。 7天后对应于潜在的纤维素降解酶GH12，GH45，裂解多糖单加氧酶和纤维二糖脱氢酶的肽最丰富。酶的这种顺序产生提供了与超孔孢梭菌的选择性木质素分解相一致的机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号