Enzymes Produced by a Pseudomonas Species Which Inactivate Inhibitors of Certain Viral Hemagglutinins. I. Identification and Purification of a Proteinase and Phospholipase C

Nathalie J. Schmidt; Pinkie S. Gee; Juanita Dennis; Edwin H. Lennette

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Enzymes Produced by a Pseudomonas Species Which Inactivate Inhibitors of Certain Viral Hemagglutinins. I. Identification and Purification of a Proteinase and Phospholipase C

机译：由假单胞菌属物种产生的酶，可以使某些病毒血凝素的抑制剂失活。 I.蛋白酶和磷脂酶C的鉴定和纯化

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Filtrates from cultures of a psychrophilic Pseudomonas species, which inactivate serum inhibitors of certain viral hemagglutinins, were shown to contain both lecithinase (phospholipase C) and a proteolytic enzyme with elastase activity. The bacterium was cultivated under conditions favoring production of the respective enzymes, and the enzymes were purified by ammonium sulfate precipitation followed by column chromatography or by gel filtration. The elastase was obtained in crystalline form and was recrystallized. It has properties similar to those of a number of other bacterial elastases but is more heat-labile than most. Although a high degree of purification was achieved for the lecithinase, as evidenced by an increase in specific activity, it was not obtained in crystalline form. Partially purified preparations of the lecithinase had extremely high activity compared to that of commercial preparations of phospholipase C from Clostridium welchii.

机译：嗜冷假单胞菌菌种的滤液使某些病毒血凝素的血清抑制剂失活，显示含有卵磷脂酶（磷脂酶C）和具有弹性蛋白酶活性的蛋白水解酶。在有利于产生各种酶的条件下培养细菌，并通过硫酸铵沉淀，然后通过柱色谱或通过凝胶过滤来纯化酶。获得了结晶形式的弹性蛋白酶，并将其重结晶。它具有与许多其他细菌弹性蛋白酶相似的特性，但比大多数细菌更不稳定。尽管卵磷脂酶可实现高度纯化，如比活性增加所证明，但并非以结晶形式获得。与来自魏氏梭状芽胞杆菌的磷脂酶C的商业制剂相比，部分纯化的卵磷脂酶制剂具有极高的活性。

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《Applied Microbiology》 |1969年第3期|共9页
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Nathalie J. Schmidt; Pinkie S. Gee; Juanita Dennis; Edwin H. Lennette;
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1. Enzymes Produced by a Pseudomonas Species Which Inactivate Inhibitors of Certain Viral Hemagglutinins [J] . Edwin H. Lennette, Juanita Dennis, Nathalie J. Schmidt, The journal of immunology . 1971,第6期

机译：由假单胞菌种产生的可灭活某些病毒血凝素抑制剂的酶
2. SB-253514 and Analogues; Novel Inhibitors of Lipoprotein-Associated Phospholipase A_2 Produced by Pseudomonas fluorescens DSM 11579 I. Fermentation of Producing Strain, Isolation and Biological Activity [J] . THIRKETTLE JAN The Journal of Antibiotics: An International Journal . 2000,第7期

机译：SB-253514和类似物;荧光假单胞菌DSM 11579产生的脂蛋白相关磷脂酶A_2的新型抑制剂I.发酵菌株的生产，分离和生物学活性
3. WA8242A(1), A(2) and B, novel secretory phospholipase A(2) inhibitors produced by Streptomyces violaceusniger - I. Taxonomy, production and purification [J] . Yoshimura S., Tsurumi Y., Muramatsu Y., The Journal of Antibiotics: An International Journal . 1998,第1期

机译：WA8242A（1），A（2）和B，由紫链霉菌产生的新型分泌型磷脂酶A（2）抑制剂-I.分类，生产和纯化
4. Study on the Endogenous Proteinase of Specially Inactivating Trypsin Inhibitors in Soybeans [C] . Guo Ling, Huo Guicheng MOST-USDA Workshop on Agricultural Products Processing and Food Safety; 20050921-24; Beijing(CN) . 2005

机译：大豆中特殊失活胰蛋白酶抑制剂的内源蛋白酶研究
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机译：第一部分：杜卡霉素SA烷基化亚基的溶剂分解区域选择性和X射线结构。第二部分靶向促红细胞生成素受体的化合物库的溶液相合成。第三部分通过阻断蛋白酶MMP2和整联蛋白α（v）beta（3）的相互作用来识别新型血管生成抑制剂
6. Enzymes Produced by a Pseudomonas Species Which Inactivate Inhibitors of Certain Viral Hemagglutinins. I. Identification and Purification of a Proteinase and Phospholipase C [O] . Nathalie J. Schmidt, Pinkie S. Gee, Juanita Dennis, 1969

机译：由假单胞菌属物种产生的酶可以使某些病毒血凝素的抑制剂失活。 I.蛋白酶和磷脂酶C的鉴定和纯化
7. WA8242A1, A2 and B, Novel Secretory Phospholipase A2 Inhibitors Produced by Streptomyces violaceusniger. I. Taxonomy, Production and Purification. [O] . SEIJI YOSHIMURA, TAKANAO OTSUKA, YASUHISA TSURUMI, 1998

机译：WA8242A1，A2和B，新型分泌磷脂酶A2抑制剂由Streptomyces violaceusniger产生。 I.分类，生产和纯化。
8. 'In vitro' Studies on the Interaction of Rickettsia and Macrophages. I. Effect of Ultraviolet Light on 'Coxiella burnetii' Inactivation and Macrophage Enzymes: UV-Inactivated 'C. burnetii'/Macrophage Enzymes [R] . Little, J. S., Kishimoto, R. A., Canonico, P. G. 1979

机译：立克次体与巨噬细胞相互作用的“体外”研究。 I.紫外线对'Coxiella burnetii'灭活和巨噬细胞酶的影响：紫外线灭活'C. burnetii'/巨噬细胞酶

Enzymes Produced by a Pseudomonas Species Which Inactivate Inhibitors of Certain Viral Hemagglutinins. I. Identification and Purification of a Proteinase and Phospholipase C

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