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首页> 外文期刊>Applied Microbiology >Virus in Water I. A Preliminary Study on a Flow-Through Gauze Sampler for Recovering Virus from Waters
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Virus in Water I. A Preliminary Study on a Flow-Through Gauze Sampler for Recovering Virus from Waters

机译：水中的病毒I.用于从水中回收病毒的流通式纱布采样器的初步研究

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摘要

A preliminary study was carried out on evaluating a flow-through gauze sampler for its efficiency in recovering virus from both fresh and seawater. An attenuated type 1 poliovirus was used as the working model. When tap water was sampled, the amounts of virus adsorbed by the gauze pads were very small, about 2% of the total number of virus particles flowing through the device. The virus adsorption and recovery increased to 15 to 19% when seawater was sampled. Addition of NaCl to tap water produced a much better effect on virus adsorption and recovery by this device, i.e., 47% of the total virus particles in each sample. The best viral elution from the pads was obtained by using buffer solution of pH 8.0 to 9.0 containing a small amount of animal serum. Repeated elutions from the pads were necessary to recover the most virus although the first eluate contained approximately 50% of the adsorbed virus. Further development of this device appears warranted, because of (i) the simplicity of the procedure, (ii) its capability of sampling large volume of water, (iii) the low cost of collecting samples, and (iv) the feasibility of obtaining a rough quantitative assessment of viral pollutants in water examined.
机译：进行了初步研究，以评估流通式纱布采样器从淡水和海水中回收病毒的效率。使用减毒的1型脊髓灰质炎病毒作为工作模型。当对自来水进行采样时，纱布垫吸附的病毒数量非常少，约占流过该设备的病毒颗粒总数的2％。取样海水后，病毒的吸附和回收率增加到15％至19％。将NaCl添加到自来水中对病毒的吸附和回收产生了更好的效果，即每个样品中病毒颗粒总数的47％。使用含有少量动物血清的pH 8.0至9.0缓冲溶液，可以从垫中获得最佳的病毒洗脱效果。尽管最初的洗脱液含有大约50％的吸附病毒，但要从病毒垫中反复洗脱是回收大多数病毒所必需的。由于（i）该程序的简单性，（ii）其对大量水进行采样的能力，（iii）收集样品的成本低以及（iv）获得水的可行性，因此该设备的进一步开发似乎是必要的。对所检查水中病毒污染物的粗略定量评估。

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