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Rapid Infrared Determination of the Potency of Chlorinated Bactericides

机译:快速红外测定氯化杀菌剂的效力

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A rapid infrared reflectance method for evaluating the germicidal potency of synthetic materials containing various amounts of two chlorinated bactericides was developed. The dimeric product 2,2′-methylenebis (4,6-dichlorophenol) exhibited a characteristic C=C skeletal inplane stretching infrared absorption band at 1,640 cm?1. The monomeric 2,4-dichlorophenol precursor showed a characteristic absorption band at 1,579 cm?1. These characteristic infrared absorptions may be used for analysis of the potency of the manufactured chlorinated bactericide. For a series of samples known to vary in dimer content, the micrograms per milliliter required for a 100% bacterial kill is first determined by a standard American Petroleum Institute method. Then the area ratio of the infrared absorption bands characteristic of the chlorinated bactericides is measured for each sample and plotted versus the microgram per milliliter required for 100% bacterial kill. The potency of subsequent samples is simply and rapidly determined by measuring this ratio from the infrared absorption curve and calculating micrograms per milliliter required for 100% kill from the calibration curve. Analysis time is approximately 1 hr compared to biocidal tests in current use requiring approximately a 1-month incubation period.
机译:开发了一种快速红外反射法,用于评估含有各种数量的两种氯化杀菌剂的合成材料的杀菌力。二聚产物2,2′-亚甲基双(4,6-二氯苯酚)在1640cm-1处显示出特征性的C = C骨架面内拉伸红外吸收带。单体2,4-二氯苯酚前体在1579cm-1处显示出特征吸收带。这些特征性的红外吸收可以用于分析所制造的氯化杀菌剂的效力。对于已知二聚体含量不同的一系列样品,首先要通过标准的美国石油学会方法确定100%的细菌杀死所需的每毫升微克。然后,测量每个样品的氯化杀菌剂特征的红外吸收带的面积比,并将其与100%细菌杀死所需的每毫升微克作图。通过从红外吸收曲线测量该比率并从校准曲线计算100%杀灭所需的每毫升微克,可以简单而快速地确定后续样品的效力。与目前需要大约1个月潜伏期的杀菌测试相比,分析时间约为1小时。

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