Microtissue Culture Test for the Titration of Low Concentrations of Diphtheria Antitoxin in Minimal Amounts of Human Sera

Marc Quevillon; André Chagnon

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Microtissue Culture Test for the Titration of Low Concentrations of Diphtheria Antitoxin in Minimal Amounts of Human Sera

机译：微组织培养试验以最低量的人血清滴定低浓度的白喉抗毒素

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A microtissue culture method for the assay of low concentrations of diphtheria antitoxin in human sera has been developed, using a monkey kidney cell (VERO) culture technique. Results obtained with sera from nonvaccinated children and with immune sera from children vaccinated with three and four injections of diphtheria pertussis tetanus vaccine were in agreement with antitoxin levels considered necessary to denote immunity to diphtheria. The use of microplates and organic buffer for culturing the animal cells improved the stability of the tissue culture system. The described method is sensitive, economical, and applicable for the titration of antitoxin in human sera particularly from infants and children from whom a minimum amount of serum is available.

机译：使用猴肾细胞（VERO）培养技术，已经开发出一种用于检测人血清中低浓度白喉抗毒素的微组织培养方法。未接种疫苗的儿童血清和接种三，四次百日咳破伤风破伤风疫苗接种儿童的免疫血清结果与认为对白喉免疫力所必需的抗毒素水平一致。使用微孔板和有机缓冲液培养动物细胞改善了组织培养系统的稳定性。所描述的方法灵敏，经济并且可用于滴定人血清中的抗毒素，特别是从可从中获得最少血清的婴儿和儿童中。

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《Applied Microbiology》 |1973年第1期|共4页
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Marc Quevillon; André Chagnon;
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1. Determination of low tetanus or diphtheria antitoxin titers in sera by a toxin neutralization assay and a modified toxin-binding inhibition test [J] . D. Sakauchi, J.f. Morais Brazilian Journal of Medical and Biological Research . 2007,第1期

机译：通过毒素中和测定和改良的毒素结合抑制试验确定血清中的破伤风或白喉低毒素滴度
2. Determination of low tetanus or diphtheria antitoxin titers in sera by a toxin neutralization assay and a modified toxin-binding inhibition test [J] . Sonobe M.H., Trezena A.G., Guilhen F.B., Brazilian Journal of Medical and Biological Research . 2007,第1期

机译：通过毒素中和测定和改良的毒素结合抑制试验确定血清中的破伤风或白喉低毒素滴度
3. The Use of the Chick in Titration of Diphtheria Antitoxin [J] . Marion F. Wormald, Sara E. Branham The journal of immunology . 1954,第6期

机译：小鸡在滴定白喉抗毒素中的应用
4. Engineering 3D muscle microtissues by co-culturing human myoblasts and fibroblasts [C] . Benoit Kalman, Claire Monge, Catherine Picart, World biomaterials congress . 2016

机译：通过共同培养人成肌细胞和成纤维细胞来工程化3D肌肉微组织
5. Influence of dairy product consumption on conjugated linoleic acid (CLA) concentration in human milk and modulation of MCF-7 human breast cancer cell signal transduction by CLA in culture [D] . Park, Yongsoon 1998

机译：乳制品消费对人乳中共轭亚油酸（CLA）浓度的影响以及培养物中CLA对MCF-7人乳腺癌细胞信号转导的调节
6. Microtissue Culture Test for the Titration of Low Concentrations of Diphtheria Antitoxin in Minimal Amounts of Human Sera [O] . Marc Quevillon, André Chagnon 1973

机译：微组织培养法测定微量人血清中低浓度白喉抗毒素的滴定
7. Determination of low tetanus or diphtheria antitoxin titers in sera by a toxin neutralization assay and a modified toxin-binding inhibition test [O] . M.H. Sonobe, A.G. Trezena, F.B. Guilhen, 2007

机译：毒素中和测定测定血清中的低伤肿或白喉抗毒素滴度和改性毒素结合抑制试验
8. A Study of the Qualitative and Quantitative Correlation of the Results of the Hi Test, the Neutralization Test in Tissue Culture, and the Agar Gel Diffusion Test in the Study of Human Sera from West Nile Fever Foci in Azerbaydzhan. [R] . Izmailova, S. P., Rubin, S. G. 1970

机译：阿塞拜疆西尼罗热病灶人体血清研究中Hi试验，组织培养中和试验和琼脂凝胶扩散试验的定性和定量相关性研究。

Microtissue Culture Test for the Titration of Low Concentrations of Diphtheria Antitoxin in Minimal Amounts of Human Sera

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