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Tissue Explants Used for Enhancement of Herpesvirus Isolations

机译：用于增强疱疹病毒分离的组织外植体

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摘要

Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made from these same tissue sources. There is also evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.
机译：将正常和实验感染的猿猴组织从准备成10％到20％匀浆的猿猴组织中回收的疱疹病毒与通过移植这些相同组织而获得的疱疹病毒进行了比较。到目前为止，外植过程是这两种方法中最敏感的。结果，通过常规病毒分离程序获得了8株疱疹病毒分离株，而从这些相同组织来源制备外植体的分离株则为74株。也有证据表明外植体方法比使用组织匀浆来恢复其他病毒更为灵敏。

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