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首页> 外文期刊>Applied Microbiology >Compatibility of Trypsin-Modified Human Erythrocytes in the Rubella Hemagglutination-Inhibition Test Employing Three Serum Treatment Procedures
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Compatibility of Trypsin-Modified Human Erythrocytes in the Rubella Hemagglutination-Inhibition Test Employing Three Serum Treatment Procedures

机译:三种血清处理程序对风疹血凝抑制试验中胰蛋白酶修饰的人类红细胞的相容性

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Results of comparative tests using trypsin-modified human type O erythrocytes and cells from newly hatched chickens with three standard serum treatment methods for rubella hemagglutination-inhibition techniques are reported. The kaolin, heparin-manganous chloride and dextran sulfate-calcium chloride methods could all be used with both cell types. Reproducibility with heparin-manganous chloride and dextran sulfate-calcium chloride was excellent with both cell types. Both methods gave generally higher antibody titers than the kaolin procedure. However, the use of human cells resulted in a more sensitive system than chicken cells with all serum treatment methods.
机译:报道了使用胰蛋白酶修饰的人O型红细胞和新孵化的鸡的细胞,用三种标准血清处理方法对风疹血凝抑制技术进行比较测试的结果。高岭土,肝素-氯化锰和硫酸葡聚糖-氯化钙方法均可用于两种细胞类型。在两种细胞类型中,肝素-氯化锰和硫酸葡聚糖-氯化钙的重现性均极佳。两种方法通常比高岭土方法产生更高的抗体效价。但是,与所有血清处理方法相比,人类细胞的使用导致了比鸡细胞更敏感的系统。

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