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首页> 外文期刊>Applied Microbiology >Relationship of Cellular Fatty Acid Composition to Survival of Lactobacillus bulgaricus in Liquid Nitrogen
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Relationship of Cellular Fatty Acid Composition to Survival of Lactobacillus bulgaricus in Liquid Nitrogen

机译：细胞脂肪酸组成与保加利亚乳杆菌在液氮中存活的关系

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Concentrated cultures of Lactobacillus bulgaricus were prepared by resuspending cells grown in semisynthetic media in sterile 10% non-fat milk solids. The concentrated cultures were frozen in liquid nitrogen for 24 h. The cell suspensions exhibited decreased viability after storage, and the amount of death varied among the different strains tested. Storage stability of all strains examined was improved by supplementing the growth medium with sodium oleate. Radioisotopes were used to study the fate of sodium oleate with L. bulgaricus NCS1. [1-14C]sodium oleate was incorporated solely into the lipid portion of the cells, including both neutral and polar lipids. The fatty acid composition of L. bulgaricus NCS1, NCS2, NCS3, and NCS4 grown with and without sodium oleate was studied. The major fatty acids of strains NCS1, NCS2, and NCS3 grown without sodium oleate were dodecanoic, tetradecanoic, hexadecanoic, hexadecenoic, and octadecenoic acids. In addition to these, strain NCS4 contained C19 cyclopropane fatty acid. The major fatty acids of all strains grown with sodium oleate were tetradecanoic, hexadecanoic, hexadecenoic, octadecenoic, and C19 cyclopropane fatty acids. All strains grown in broth containing sodium oleate contained larger amounts of octadecenoic and C19 cyclopropane fatty acid, and less saturated fatty acids than when grown without sodium oleate. Statistical analyses indicated that C19 cyclopropane fatty acid was most closely related to stability of the lactobacilli in liquid nitrogen. A negative regression line that was significant at P < 0.001 was obtained when the cellular content of this fatty acid was plotted against death.
机译：通过将在半合成培养基中生长的细胞重悬在无菌的10％脱脂乳固体中，制备保加利亚乳杆菌的浓缩培养物。将浓缩的培养物在液氮中冷冻24小时。储存后，细胞悬浮液的活力降低，并且死亡的量在所测试的不同菌株之间变化。通过向生长培养基中补充油酸钠可以改善所有检测菌株的储存稳定性。放射性同位素用于研究油酸钠与保加利亚乳杆菌NCS1的命运。 [1-14C]油酸钠仅掺入细胞的脂质部分，包括中性和极性脂质。研究了在有或没有油酸钠的情况下生长的保加利亚乳杆菌NCS1，NCS2，NCS3和NCS4的脂肪酸组成。在没有油酸钠的情况下生长的菌株NCS1，NCS2和NCS3的主要脂肪酸为十二烷酸，十四烷酸，十六烷酸，十六烯酸和十八烯酸。除了这些，菌株NCS4还包含C19环丙烷脂肪酸。用油酸钠生长的所有菌株的主要脂肪酸为十四烷酸，十六烷酸，十六烯酸，十八烯酸和C19环丙烷脂肪酸。与不含油酸钠的培养相比，在含油酸钠的肉汤中生长的所有菌株均含有大量的十八烯酸和C19环丙烷脂肪酸，饱和脂肪酸较少。统计分析表明，C19环丙烷脂肪酸与液氮中乳酸菌的稳定性最密切相关。当绘制该脂肪酸的细胞含量相对于死亡的图时，获得的负回归线在P <0.001时显着。

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